Anti-inflammatory Effect Of Low-level Laser Therapy On Infectious Endophthalmitis In Rabbits

Infectious endophthalmitis is the most serious complication after intraocular surgery and penetrating eye injuries. Infectious endophthalmitis can quickly damage eye tissue and visual function, and results in loss of vision and ocular atrophy if the inflammation is not effectively controlled. It is now generally believed that tissue damage and intraocular inflammation are closely related. Therefore, anti-inflammatory treatment is as important as anti-microbial treatment to control disease progression and protect visual function. Dexamethasone is widely used to treat endophthalmitis. However, some researchers believe that dexamethasone can damage the immune system, and may accelerate intraocular infection and the destruction of intraocular tissue.In recent years, low-level laser therapy (LLLT) has been widely applied in medicine. Many studies have found that LLLT has a significant biological regulatory role in the inflammatory process, such as the ability to reduce the speed and extent of inflammatory exudation, improved capillary permeability to promote absorption and dissipation of inflammation, etc.ObjectiveIn this study, a rabbit endophthalmitis model for Staphylococcus epidermidis infection was established, and either LLLT or dexamethasone was administered in combination with vancomycin to determine the anti-inflammatory effect of LLLT on infectious endophthalmitis and to assess the feasibility of LLLT to reduce or replace dexamethasone in the treatment of infectious endophthalmitis.Methods1. Different dose laser exposured to rabbit’s retina through pupil, then routine ophthalmitic examination and F-ERG were practiced in left rabbit eyes to investigate the safty of LLLT.2. To inject inactived staphylococcu aureus into rabbits’ left eyes and establish endophthamitis model. LLLT was used to relieve inflammation. To investigate the effectivity of LLLT in infectious endophthalmitis (P<0.05).3. To inject staphylococcu epidermidis into rabbits’left eyes and establish endophthamitis model. With the injection of Vancomycin into vitreous dexamethasone and LLLT was usded to relieve inflammation (P<0.05).4. To inject Aspergillus fumigatus into rabbits’left eyes and establish endophthamitis model. With the injection of Amphotericin B into vitreous cavity to antisepticize, dexamethasone and LLLT was usded to relieve inflammation.5. LLLT expoure to HL-60 provocatived by PMA, the concertration of TNF in the supernatant solution was determined by ELISA.Results:1. Suitable dose LLLT is safe. The results of F-ERG didn’t show the inreversible damage of retina (P<0.05).2. LLLT relieved the inflammmation obviosly of the endophthlmitis induced by bacteriotoxin and protected the function of retina (P<0.05).3. With the effect of vancomycin, LLLT and dexamethasone showed the obvious anti-inflammation role to Staphylococcus epidermidis endophthalmitis at different time (P<0.05).4. Established the Aspergillus fumigatus endophthalmitis successfully, the inflammation of Aspergillus fumigatus endophthalmitis was very strong and developed panophthalmitis in a short time, so it was difficult to investigate the anti-inflammation of LLLT to Aspergillus fumigatus endophthalmitis.5. The concertration of TNF-αproduced by HL-60 expoured to LLLT was lower (P>0.05).Conclusion:LLLT showed a good anti-inflammation in endophthalmitis induced by inactived staphylococcu aureus or staphylococcu epidermidis. LLLT can be used to treat infectios endophthalmitis.