Neuroglobin Attenuates Alzheimer-like Tau Hyperphosphorylation By Activating Akt Signaling

Neuroglobin (Ngb) is a recently identified member of hemoglobin family, distributed mainly in central and peripheral nervous systems. Recent studies suggest that Ngb can protect neural cells from?-amyloid-induced toxicity in Alzheimer disease (AD). Hyperphosphorylation of tau is another characterized pathological hallmark in the AD brains, however, it is not reported whether Ngb also affects tau phosphorylation. Here, we found that the level of Ngb was significantly reduced in Tg2576 mice (a recognized mouse model of AD) and TgMAPt mice, and the level of Ngb was negatively correlated with tau phosphorylation. Overexpression of Ngb attenuates tau hyperphosphorylation at multiple AD-related sites induced by upregulation of glycogen synthase kinase-3?(GSK-3?), a crucial tau kinase. While Ngb activates Akt and thus inhibits GSK-3?, simultaneous inhibition of Akt abolishes the effects of Ngb on GSK-3?inhibition and tau hyperphosphorylation. Our data indicate that Ngb may attenuate tau hyperphosphorylation through activating Akt signaling pathway, implying a therapeutic target for AD.