Study On Modified San Jia Powder And Its Sub-Formulas Of Yin-Blood-Enriching Regulating TGF-?/Smads Signaling Pathway Of Hepatic Fibrosis

Objective:To clarify the mechanism of Modified San Jia Powder and its Sub-Formulas(Yin-blood-enriching) on hepatic fibrosis by observing the Effects of them on TGF-?/Smads signaling pathway of rats with hepatic fibrosis.Methods:copying the hepatic fibrosis model in rats by CCl4, and using TUNEL?RIA?SP and in situ hybridization technique to inquire the mechanism of Modified San Jia Powder and Its Sub-Formulas on Hepatic Cell Apoptosis of Rats with Hepatic Fibrosis.Results:1. Modified San Jia Powder is helpful to improve the general health situation of rats in terms of activity, appetite, skin color, luster, weight and Pathomorphology. It can decrease the level of P?NP,HA and Apoptosis(P< 0.01), can inhibit the expression of TGF-?1 (P<0.01), Smad2/3 (P<0.01), PAI-1 (P< 0.05) and a2 (I) precollagen mRNA (P<0.01)?2. The group of Yin-blood-enriching is helpful to improve the general health situation of rats in terms of activity, appetite, skin color, luster, weight and Pathomorphology. It can decrease the level of Apoptosis(P<0.01), can inhibit the expression of Smad2/3 (P<0.01), and can decrease the level of PIIINP,HA, inhibit the expression of TGF-?1, PAI-1 and a2 (I) precollagen mRNA with herbs of activating blood and dissolving stasis and of expelling Evil.Conclusions:1. The effects of Modified San Jia Powder:Modified San Jia Powde can improve the rats`general health situation and reduce the fibrotic degree of rats hepatic tissue to protect and treat hepatic fibrosis. The result of Modified San Jia Powder is better than herbs of Yin-blood-enriching. 2. The mechanism of Modified San Jia Powder:Modified San Jia Powder can treat hepatic fibrosis by decreasing the level of P?NP,HA and Apoptosis, inhibiting the expression of TGF-?1, Smad2/3, PAI-1 and a2 (?) precollagen mRNA.3. The effects of Yin-blood-enriching:The method of Yin-blood-enriching can slightly improve the rats' general health situation and Pathomorphology to treat hepatic fibrosis.4. The mechanism of Yin-blood-enriching:The method of Yin-blood-enriching can treat hepatic fibrosis by decreasing the level of Apoptosis, inhibiting the expression of Smad2/3. It also can treat hepatic fibrosis by inhibiting the expression of TGF-?1, PAI-1 and a2 (?) precollagen mRNA with herbs of activating blood and dissolving stasis and of expelling Evil.