| In order to improve the phenomenon of exercise fatigue that often occurs in horse racing activities,the research team studied the improvement of acupoint catgut embedding preconditioning on exercise endurance of exercise-induced fatigue model rats.Sports endurance can be effectively improved.In this experiment,the combined analysis of transcriptome and metabolome,detection of serum lipid metabolism indexes,pathological slices,q-PCR and western blot methods are used to explore the mechanism of acupoint catgut embedding on exercise-induced fatigue rat models.The results are as follows:(1)In order to study the changes of m RNA in exercise-induced fatigue model rats after acupoint catgut embedding preconditioning,transcriptome sequencing was used to analyze the sequencing results.A total of 32623 genes were identified after catgut embedding at acupoints.Compared with the model group,363 differentially expressed genes were identified in group ?.Among the differentially expressed genes,144 were up-regulated and 219 were down-regulated.A total of 458 differentially expressed genes were identified between the catgut embedding group?and the model group.Among the differentially expressed genes,248 genes were up-regulated and 210 genes were down-regulated.Through KEGGpathway enrichment analysis,it is found that acupoint catgut embedding can improve exercise endurance of rat model of exercise-induced fatigue through negative regulation of apoptosis,improvement of redox process,regulation of skeletal muscle differentiation and regulation of lipid metabolism through PPAR signal pathway.(2)In order to study the changes of metabolites in exercise-induced fatigue model rats after acupoint catgut embedding preconditioning,the metabonomic sequencing method was used to analyze the results.Acupoint catgut embedding can up-regulate the content of arachidonic acid in exercise-induced fatigue model rats.Through differential metabolite enrichment signal pathway,it was found that arachidonic acid metabolic pathway,PPAR signal pathway and biosynthesis metabolic pathway of unsaturated fatty acids play an important role in acupoint catgut embedding treatment of exercise-induced fatigue rat model.(3)In order to study the difference of fat metabolism between exercise fatigue model rats and acupoint catgut embedding preconditioning exercise fatigue model rats,the contents of TG,TC,LDH,HDL,ALT,AST,GLU,FFA and AA in serum were determined by serum biochemical test and ELISA method,and the histology of quadriceps femoris and liver in rats were studied.The results showed that exercise-induced fatigue could significantly decrease the contents of TG and TC in serum of rats.The contents of LHD,ALT and AST increased significantly,while the contents of GLU,FFA and AA decreased significantly.After acupoint catgut embedding pretreatment,the contents of FFA and AA in serum were significantly increased,while the contents of TG,LDH and AST were significantly decreased.Histological observation of quadriceps femoris and liver tissue sections showed that acupoint catgut embedding pretreatment could promote the repair of tissue damage caused by exercise-induced fatigue.(4)In order to elucidate the mechanism of acupoint catgut embedding on the regulation of lipid metabolism in rat model of exercise-induced fatigue,the expressions of SLC27A2,FABP1,APOC3 and LPL in quadriceps femoris and liver were detected by q PCR and western-blot methods.The results showed that the rat model of exercise-induced fatigue could up-regulate the expression of SLC27A2,FABP1,APOC3 and LPL in quadriceps femoris and liver.Compared with the model group,after acupoint catgut embedding pretreatment,the expression of SLC27A2,FABP1 and APOC3 in quadriceps femoris and liver tissue was down-regulated,while the expression of LPL was up-regulated.Conclusion:(1)Regulating lipid metabolism through PPAR signal pathway is one of the methods to improve exercise endurance of rat model of exercise-induced fatigue by catgut embedding at acupoint.(2)Serological indexes and pathological results show that acupoint catgut embedding can not only promote fat decomposition and reduce lactic acid accumulation,but also promote the repair of tissue injury and liver injury caused by exercise-induced fatigue.(3)Catgut embedding at acupoint regulates PPAR signal pathway by up-regulating the expression of LPL and down-regulating the expression of SLC27A2,FABP1 and APOC3 to further improve fat metabolism.(4)In clinical work,the selected acupoints of catgut embedding group I are recommended to improve exercise endurance. |
PDF Full Text Request