Physiological Roles Of Urea Carboxylase Gene And Allophanate Hydrolase In Bemisia Tabacci

Nitrogen is one of the basic elements of lives.In metabolism,organisms converted toxic nitrogen-contianing products such as ammonia and nitrogen oxides into final nitrogenous waste and excreted them out of body.Through final nitrogenous waste synthesis,life can effectively avoid damage which caused by toxic nitrogen-containing compounds.As a result,final nitrogenous waste biosynthesis is important in nitrogen metabolism.Besides final nitrogenous waste biosynthesis,nitrogen recycling is also reported to be an important part of nitrogen cycle in some insects,such as cockroach,termites.When faced with nitrogen insufficient supplyment,some insects maintian their own growth and development by recovering nitrogen from nitrogen waste.As a typical sucking insect,B.tabaci MED excreted amino acid rather than uric acid.Furthermore,B.tabaci MED haas been reported to be effective in coping with nitrogen deficiencies in food.As a result,it seem that the B.tabaci MED harborred a unique nitrogen metabolism.In our study,two bacteria origined horizontial transferred genes,urea carboxylase(BtQUca)and allophanate hydrolase(BtQAp)of B.tabaci MED were focused to investigate the roles of the unique nitrogen metabolism of B.tabaci MED.Functional study results showed these two genes still retained their function of urea degradation.In addition,our results also demonstrated these two gene not only accounted for the unique nitrogenous waste biosynthesis of B.tabaci MED,but also built nitrogen recycling of B.tabaci MED.Our study showed these two genes were key constructors of nitrogen metabolism of B.tabaci MED.Above all,unique nitrogen metabolism of B.tabaci MED was revealed via investigating the roles of two HTGs.Our study provides a theroetical basis for revealing the powerful adaptive mechanism of B.tabaci and explaining the nitrogen metabolism mechanism of sucking insects.