| Maize ear rot is one of the world’s widely occur within the scope of diseases.In our country,maize is the main food crops,due to the ongoing maize ear rot,seriously affecting the yield and quality of maize,including Fusarium moniliforme is the main pathogenic bacteria infection of maize ear rot,its toxic secondary metabolites mycotoxins,would be a serious threat to human and animal health,and even cause food safety problems.As people environmental protection consciousness enhancement and concern about food safety,the safe,pollution-free with more attention paid to the biological control,inhibitory action is friendly to environment,safety,the advantages of pathogen specificity is strong can effectively control the plant disease.Based on the solution of Bacallus amylolaquefaciens HRH317 starch as the inhibitory action,maize ear rot for pathogen,Fusarium moniliforme research maize seedling stage,HRH317,the inhibitory action on the inhibition mechanism of Fusarium moniliforme,inhibitory action for further utilization of biological control to provide theoretical basis for maize ear rot disease.In this paper,the main research results are as follows:1.Oxford cup method is adopted to improve the solution of B.amylolaquefaciens HRH317 bacteriostatic experiment of Fusarium moniliforme,bacteriostatic ring size and inhibitory action to bacillus HRH317 growth as an index,the fermentation culture medium and fermentation conditions were optimized,the use of statistical methods,based on the single factor and orthogonal test and the response surface analysis,to determine the optimum fermentation culture medium formula for glucose 2.0%,peptone 0.5%,yeast powder 1%,0.5% sodium chloride,magnesium sulfate 1.0% and optimum fermentation conditions for the fermentation time of 25.1 h,37 ℃ fermentation temperature,rotating speed of 166 r/min,under the condition of the bacteriostatic circle diameter size has never before optimization of26.36 mm to 33.31 mm,bacteriostatic activity increased by 26.37%.2.Through the pot experiment of maize,maize to 3 leaf at seedling stage growth period of maize leaf as the research object,by seed saturation method inoculated pathogen Fusarium moniliforme inhibitoryaction and B.amylolaquefaciens HRH317,contrast after vaccination inhibitory action of different processing conditions,the maize leaves department of defense enzyme Fusarium moniliforme stress response and antagonism resistance mechanism of the interactions,the results showed that the inhibitory action alone inoculation maize seeds with HRH317,the superoxide dismutase(SOD),catalase(CAT),phenylalanine ammonia enzyme(PAL),peroxidase(POD),polyphenol oxidase(PPO),chitinase,β-1,3glucanase are all in a certain degree of entrainment and maize resistant ability,can be induced by inoculation and inoculation of pathogen,the inhibitory action of maize seeds,pathogenic bacteria and inoculation treatment at the same time,the inhibitory action,induction of SOD,CAT and PAL and POD ability is stronger,and PPO,chitinase and β-1,3 glucanase showed the enzyme activity induced ability are relatively weak,presumably HRH317 induced defense related enzyme activity,the inhibitory action,is to reduce or prevent disease,and play the role of antagonism disease suppression is an important way.3.The test through four different ways of maize seed treatment,using the HPLC determination of maize seedling stage in different time of maize leaf fumonisin B1 content,and carries on the dynamic analysis,the results showed that the maize growth and trilobites period,after prolonged,with growth solution under different conditions of B.amylolaquefaciens HRH317 to a certain extent,inhibit fumonisin B1,reduce fumonisin B1 content,further illustrate HRH317,the inhibitory action of maize ear rot disease caused by Fusarium moniliforme biological control agents.4.Using scanning electron microscope(sem),transmission electron microscope(tem)and fluorescence microscope,the cellular and subcellular level,B.amylolaquefaciens HRH317 research solutions to Fusarium moniliforme mycelium morphology and ultrastructure of Fusarium moniliforme,the results show that the different processing time,Fusarium moniliforme mycelium are different levels of cracking,the fracture phenomenon is serious,with the extension of time hypha cell membrane damage,organelles is disorder,damage and degradation,the Fusarium moniliforme growth is restrained,further for B.amylolaquefaciens HRH317 against Fusarium moniliforme infection inhibitory mechanisms to provide theoretical basis for the research. |
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