Gene Cloning And Molecular Characterization On Swine Epidemic Hepatitis Virus

Hepatitis B Virus and Hepatitis E Virus are two important zoonotic diseases that have spreaded widely in developing countries and threaten the health of both animals and human beings, great economic loss have been caused by these diseases. Hepatitis B Virus and Hepatitis E Virus usually get co-infected in modern hog pen, the incidence turned out to be growing these years. To investigate the pathogenic characteristics and effectively control the diseases of HBV and HEV, a series of experiments suck as local epidemic virus isolation, epidemiology survey, gene cloning, the analysis of molecular structure, gene mapping, molecular diagnosis and immunization research were conducted in this study. The results are as follows:1 967 Serum samples from slaughterhouse, hog pen and peasant in and around Lanzhou were collected for epidemiology survey and HBV separation, The results are as follows :?The detection revealled the samples that have HbsAg, anti-HbsAg antibody, HbeAg ,anti-HbeAg antibody and anti HbcAg antibody all was 1.5%, the samples that have HbeAg alone was 5.9; the samples that contains anti-HbsAg antibody was 16%.?the anti-HbsAg antibody rate in the sample from jingyuan county was 23.2% and the anti-HbsAg antibody rate in the sample from jingtai count was 20.5%. these were the highest rate in the samples.?The samples from above 8 month pigs had more higher anti-HbsAg antibody rate than that from under 8 month pigs, the HBE and HBV rate were 14.6 and 4.5%, respectively. swCH146 shared 89.5% nt identity , which is the highest one in this comparison, with that of swCH25 strain.HEV swCH146 genome contains three ORFS. HEV swCH146 ORF1 shared 87.5% nt identity with that of type (ID11093 ), 83.2% nt identity with that of type II mexican strain(M74506.1), 91.0% nt identity with that of type III HEV SWKOR-2 strain(FJ426404) and 97.2% nt identity with that of HEV type?KNIH-hHEV4 strain(FJ763142.1) that published in Genbank, respectively. swCH146 ORF2 shares 92.4%?94.9% nt identity with that of type I, type II and type III HEV and shared 98.9% nt identity with that of HEV type?HEV that published in Genbank. swCH146 ORF3 shares 87.5%?90.6% nt identity with that of type I, type II and type III HEV and shared 98.4%. nucleotide identity with that of type?H EV that published in Genbank5 Recombinant adenovirus plasmid of ORF2 gene of HEV GS was designed, and the AD-293 cells were used for transinfection. Defective virus which was replicated by AD-E was observed by fluorescence detection. This established a good foundation for recombinant vaccines. swCH146 shared 89.5% nt identity , which is the highest one in this comparison, with that of swCH25 strain.HEV swCH146 genome contains three ORFS. HEV swCH146 ORF1 shared 87.5% nt identity with that of type (ID11093 ), 83.2% nt identity with that of type II mexican strain(M74506.1), 91.0% nt identity with that of type III HEV SWKOR-2 strain(FJ426404) and 97.2% nt identity with that of HEV type?KNIH-hHEV4 strain(FJ763142.1) that published in Genbank, respectively. swCH146 ORF2 shares 92.4%?94.9% nt identity with that of type I, type II and type III HEV and shared 98.9% nt identity with that of HEV type?HEV that published in Genbank. swCH146 ORF3 shares 87.5%?90.6% nt identity with that of type I, type II and type III HEV and shared 98.4%. nucleotide identity with that of type?H EV that published in Genbank5 Recombinant adenovirus plasmid of ORF2 gene of HEV GS was designed, and the AD-293 cells were used for transinfection. Defective virus which was replicated by AD-E was observed by fluorescence detection. This established a good foundation for recombinant vaccines.