Research Of On-line Fluorescence Detection Under High Pressure

As an important parameter, pressure has been widely used in biology research. Especially after spectacular biological communities were discovered in extreme environments such as hydrothermal vent, pressure has attracted more attentions in the research of extremophiles and biomolecule. As the use of spectroscopy method in high pressure research, the on-line detection under high pressure becames possible. Especially fluorescence spectrometry was used in high pressure research, the experiment becames more sensitive and reliable. At present, There is tremendous progress in the researches about biomolecule under pressures. These researches are very important in studing the interaction of biomolecular, the changes of biomolecular structure, and the activities of extremophiles et al. With the progress of spectrum methods under pressures, the devices of on-line detection under high pressure were developed.First, the paper summarized the researches about on-line fluorescence detecion. Then We developed an on-line fluorescence detection device which could be installed in common fluorescence spectrophotometer. This device could be used for on-line fluorescence detection under high pressure, and also could be connected with a large-scale device for flow-system detection on-line. With this system, the fluorescence spectrum of Rose Bengal under pressure was studied. The results showed that the device was valid for on-line fluorescence detection under high pressure. At the same time, the results of Rose Bengal provied important direct evidence for biomolecule researches.Second, tryptophan is an important intrinsic fluorophore in reasearches of the deep sea, so we studied the fluorescence spectrum of pure tryptophan and metal ions/tryptophan mixtures under high pressures. The results showed that the effect of pressure on pure tryptophan and metal ions/tyrptophan mixtures must be considered in researches of macromolecular under pressure.Another important section of this paper was a high pressure on-line observation system for microorganism. The system could be used with fluorescence microscope or long distance microscope. Using this system, we studied Cyclops under different pressures. This was important in researches of the distribution of plankton and activities under high pressures.