Identification and characterization of AU-rich elements and trans-acting factors involved in the post-transcriptional regulation of human interleukin-3 | Posted on:2015-08-03 | Degree:Ph.D | Type:Dissertation | University:University of Puerto Rico, Rio Piedras (Puerto Rico) | Candidate:Gonzalez Feliciano, Jose A | Full Text:PDF | GTID:1474390017499639 | Subject:Biology | Abstract/Summary: | | An important aspect of the regulation of gene expression in eukaryotes is the post-transcriptional control. Adenosine/Uridine-rich elements (AU-rich elements or AREs) within the 3'-untranslated region (3'-UTR) of mRNAs play a crucial role in the post-transcriptional regulation by affecting mRNA stability and/or translation. Cells involved in the immune response produce several pleiotropic lymphokines that contain AU-rich elements in their mRNAs. Interlukin-3 is one of these lymphokines that harbor an AU-rich element in its 3'-UTR. The aberrant expression of IL-3 is related to cancer, sickle cell disease and asthma, among others. At present, little is known about the ARE-mediated post-transcriptional regulation of human IL-3. In order to fill this scientific gap, the main focus of this dissertation is to understand the human IL-3 ARE-mediated post-transcriptional regulatory pathway. This manuscript is divided in four chapters. Chapter I consists of an introduction to the ARE-mediated post-transcriptional regulatory mechanism. Chapter II focuses on the characterization of the IL-3 ARE post-transcriptional role. Chapter III describes and characterizes the ARE-BPs that recognize the hIL-3 ARE and their roles in mRNA stability and translation. Finally, Chapter IV elucidates the role of T-cell activation in the hIL-3 ARE-mediated post-transcriptional regulation and its effects in the IL-3 ARE-binding protein complexes. Altogether, the results presented in this dissertation provide new evidence regarding the IL-3 ARE-mediated post-transcriptional regulation in human cells. | Keywords/Search Tags: | Post-transcriptional, Regulation, Au-rich elements, IL-3, Human | | Related items |
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