THE CO-MUTAGENIC POTENTIAL OF TRANS-FATTY ACIDS IN THE METABOLISM OF BENZO(A)PYRENE, 2-ANTHRAMINE, 2-ACETYLAMINOFLUORENE AND 2-AMINOFLUORENE (BENZOPYRENE)

The effects of the incorporation of trans-fatty acids in the diet of rats on the mutagenic potential of benzo(a)pyrene (BP), 2-aminofluorene (AF), 2-acetylaminofluorene (AAF) and 2-anthramine (AA) were evaluated in the Ames Assay incorporating rat hepatocytes and liver cell homogenates (S-9 fraction) as the metabolic activating systems. The hepatocytes and S-9 fractions were derived from Aroclor or BHT induced and non-induced animals fed the experimental and control diets, 45% to 55% and 0% trans-fatty acids of a 15% fat diet, respectively.;The effects of diet on mutagenesis were few. The mutagenic potential of benzo(a)pyrene was significantly decreased in the S-9 assays from Aroclor induced animals fed the trans-fatty acid diet when compared to S-9 assays from Aroclor induced animals fed the control diet. The mutagenic response from 2-acetylaminofluorene was significantly higher in S-9 assays from BHT induced animals fed the trans-fatty acid diet than S-9 assays from animals fed the control diet. In assays from non-induced animals, a higher concentration of 2-acetylaminofluorene was required for mutagenesis with S-9 from trans-fatty acid fed animals than S-9 from animals fed the control diet.;In the hepatocyte assays, benzo(a)pyrene, 2-anthramine and 2-aminofluorene did not produce a mutagenic response. Effects from the inducers Aroclor and BHT or diet on the mutagenicity of benzo(a)pyrene, 2-anthramine and 2-aminofluorene were not observed in the hepatocyte assay. The S-9 fractions were more effective than hepatocytes in the metabolic activation from benzo(a)pyrene and 2-aminofluorene.;Benzo(a)pyrene, 2-acetylaminofluorene and 2-aminofluorene were mutagenic in the S-9 assays from Aroclor and BHT induced animals fed the control and trans-fatty acid diets. In the S-9 assays from non-induced animals fed the control and trans-fatty acid diets, benzo(a)pyrene and 2-acetylaminofluorene were weakly mutagenic. Aroclor was a more effective inducer of benzo(a)pyrene and 2-acetylaminofluorene mutagenicity than BHT. The effects of the inducers, Aroclor and BHT, on the mutagenic potential of 2-aminofluorene was negligible.