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Use of Molecular Markers to Investigate Epidemiology of and Host Manipulation by the Invasive Parasite Dicrocoelium Dendriticum

Posted on:2018-01-20Degree:Ph.DType:Dissertation
University:University of Lethbridge (Canada)Candidate:van Paridon, BradleyFull Text:PDF
GTID:1473390020453537Subject:Parasitology
Abstract/Summary:
Parasite distributions are rapidly expanding, in large part due to human mediated translocations of hosts. Thus, instances of parasite-spread to new hosts are common. Management of these emerging parasites depends on understanding the host-shifts occurring within invasive areas and the pathway of invasion; both of which can be investigated using molecular markers. Dicrocoelium dendriticum is one such emerging parasite, which has invaded Alberta. Here, I use molecular markers to identify two local Albertan hosts colonized by D. dendriticum; the snail Oreohelix subrudis and the ant Formica aserva. I also developed a panel of nine microsatellite markers that showed two separate introductions of the parasite into Canada. Furthermore, the markers also identified "clumped clonal transmission" throughout the life-cycle, which has implications for the potential evolution of cooperation via kin selection, in this behaviour altering parasite.
Keywords/Search Tags:Parasite, Molecular markers, Dendriticum
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