| The initial study involved the development of an analytical procedure using high-performance liquid chromatography and the 9-fluorenylmethyl chloroformate-1-aminoadamantane (FMOC-ADAM) amino acid fluorescence-tagging technique for the determination of hydroxyproline (HYP) in very small muscle samples. A 7.5 min elution program was developed wherein HYP was completely resolved in three min. An alternate program was established for the additional determination of proline wherein it eluted after six min with a total run time of 10 min. This procedure was proven to provide results similar (P =.12) to those of a standard colorimetric assay.;In a second study, 49 Holstein beef animals, representing all USDA maturity groups, were utilized to determine the effect of carcass maturity on the content of pyridinoline, a mature collagen crosslink, in bovine intramuscular collagen (IMC) as well as the relationship of pyridinoline to thermal stability of IMC. As carcass maturity increased, heat-labile collagen solubility (% Sol) and thermal shrinkage temperature (T;In the final study, the effect of early-postmortem aging at three temperatures (1, 19 and 37... |
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