Biological disposition and in vitro activity of doxorubicin delivered in polymeric microspheres

olymeric microspheres were prepared using poly(lactic acid) (PLA), poly(glycolic acid) (PGA) and bovine serum albumin (BSA). Phenolphthalein (PT) and bromsulphthalein (BSP) were incorporated in PLA and PGA microspheres using solvent evaporation and precipitation techniques, respectively. Doxorubicin was incorporated in BSA microspheres using the emulsion technique with thermostabilization of microparticles. These microspheres were characterized in terms of particle size, morphology, surface charge, dye/drug content and in vitro dissolution. An in vivo method was developed to assess the kinetics of disposition of dye/drug in bile following release from polymeric microspheres in a rat model. Cell culture methods were used to assess the effects of the drug on cell replication.;The diameters of the microparticles were less than 10...