Effect of environmental temperature on Antarctic fish antifreeze protein activity and concentration

The Southern Ocean is an extreme cold environment with regions that can be variable in their temperature profiles. The low latitudes of the western Antarctic Peninsula (WAP) experience freezing sea surface water temperatures and ice formation only during the austral winter but the water below 100 m is warmer due to the effects of the Circumpolar Deep Water (CDW) that flows onto the shelf. During the winter the temperature gradient can range from -1.9°C at the surface to +2.0°C at depth. In contrast, the high latitude regions of the southwestern Ross Sea and McMurdo Sound can be at the freezing point of seawater (-1.9°C) nearly year-round. McMurdo Sound is bordered to the south by the Ross Ice Shelf which is a source of upwelling of supercooled water. In the WAP environmental ice crystals are found only in the surface water from surface ice formation during the winter while the southwestern Ross Sea is ice covered for much of the year.;The presence of environmental ice crystals poses a challenge for marine ectotherms. Despite this, marine life is abundant in this region. The largest group of fishes in this region is the suborder Notothenioidei. For notothenioid fishes blood serum osmolytes only depress the freezing point of their blood to about -1.0°C, almost a full degree warmer than the environmental freezing point. The success of this group in the Southern Ocean is attributable in part to the evolution of blood antifreeze proteins (AFPs) that adsorb to the surface of internalized ice crystals and inhibit their growth. The non-colligative depression of the blood serum freezing point can contribute enough protection to prevent freezing down to or below the freezing point of seawater. The evolution of AFPs is what allowed the notothenioid fishes to dominate the ichthyofaunal biomass of the Southern Ocean after freezing water temperatures caused most of the late Eocene fish fauna to go extinct. Notothenioids have two types of AFPs: antifreeze glycoproteins (AFGPs) that are comprised of glycosylated tripeptide repeats of various sizes from large size isoforms with numerous repeats to small size isoforms with relatively few tripeptide repeat sequences, and an antifreeze potentiating protein (AFPP) that potentiates the activity of the AFGPs.;The largest family within Notothenioidei is the Nototheniidae, comprised of 49 species in 13 genera. Members of this family are found in both the WAP and Ross Sea regions of the Southern Ocean.;To investigate whether populations of species within a genus differed in their AFPs and freezing points I sampled ten species from the genus Trematomus (family Nototheniidae) from both the high latitude southwestern Ross Sea and the WAP. This genus is often considered a high latitude radiation due to its abundance in both the shallow and deep water in this region. Surface temperature and environmental ice was similar in both locations but deep water temperatures differed with the southwestern Ross Sea having bottom temperatures of -1.9°C and the WAP as warm as +0.3°C.;I expanded this study to include more species from multiple genera from the WAP. Fishes were sampled during the austral winter 2014 when bottom water was between +0.5°C and -0.5°C. Five species from two of the four genera sampled are found circum-Antarctic, including three species from the genus Trematomus, the remaining three species from two genera are only found in the WAP.;I also focused on the southwestern Ross Sea where there is very little thermal variation for much of the year. I was interested in following one species with shallow water populations during the transition from freezing water temperatures through the onset of summer warming when water temperatures increased from -1.90°C to -0.31°C. High concentrations of AFGP were observed from locations where water temperatures were still at -1.9°C but concentration decreased in the late season location corresponding to the increased temperature at that sampling time.;Overall, I found that antifreeze protein concentration and activity was dependent on sampling location and temperature with populations from the southwestern Ross Sea having higher AFP activity and concentration than populations from the WAP. The same trend was seen when comparing sampling depth. Shallow water populations had higher AFP levels due to increased contact with environmental ice and deep water populations had lower AFP levels due to rarely coming into contact with environmental ice or by inhabiting deep water that could be 4°C warmer than the surface water. Species found at these warm depths but that also have circum-Antarctic distributions had higher AFP levels than species confined to warmer regions and warmer depths. (Abstract shortened by UMI.).