| A virus-induced disease causing mosaic, line pattern and concentric chlorotic ringspots on Epimedium hybrids was first reported by Nameth et al. in 1991. Preliminary analysis including host range studies, dsRNA analysis, electron microscopy, serology, and ELISA indicated that this virus belonged to the tobamovirus group with a monopartite (+) sense RNA genome. The size of the coat protein was determined to be ;Primers were designed using existing sequence information of several tobamoviruses, to anneal to a region encompassing part of the 183 K gene, the entire 30K cell-to-cell movement protein gene and part of the coat protein gene. A modified method was used to purify large quantities of ssRNA, which was reverse transcribed to form cDNA, and was directly amplified via polymerase chain reaction (RT-PCR). The amplified double stranded PCR product was directly sequenced by DyeDeoxy;The sequence contained 207 nucleotides or 69 amino acids from the 3... |
