The effect of growth surface properties on the cultivation of anchorage-dependent animal cells and the development of novel surfaces for cell culture

The physiological responses of anchorage dependent animal cells (3T6 mouse embryo fibroblast and A375 human malignant melanoma) to systematic variation of growth surface properties were determined for sulfonated polystyrene (PS-S), styrene-methacrylic acid copolymers (PS-MAA), and styrene-dimethylaminoethyl methacrylate copolymers (PS-DMAEMA). The optimal surface charge densities for attachment, spreading, and proliferation occur over narrow ranges and are not equal. On PS-S, the optimum charge densities for 3T6 are 2.5 charges/nm;A fixed-surface bioreactor based on a reticulated vitreous carbon foam was used to cultivate 3T6, providing a high surface-to-volume ratio (17 cm;The influence of inoculum density on cell attachment and growth was also studied. The initial cell density (cells/surface area) must be 10...