| The physiological responses of anchorage dependent animal cells (3T6 mouse embryo fibroblast and A375 human malignant melanoma) to systematic variation of growth surface properties were determined for sulfonated polystyrene (PS-S), styrene-methacrylic acid copolymers (PS-MAA), and styrene-dimethylaminoethyl methacrylate copolymers (PS-DMAEMA). The optimal surface charge densities for attachment, spreading, and proliferation occur over narrow ranges and are not equal. On PS-S, the optimum charge densities for 3T6 are 2.5 charges/nm;A fixed-surface bioreactor based on a reticulated vitreous carbon foam was used to cultivate 3T6, providing a high surface-to-volume ratio (17 cm;The influence of inoculum density on cell attachment and growth was also studied. The initial cell density (cells/surface area) must be 10... |
