Identification and functional characterization of active-site amino acids in mevalonate kinase

Posted on:1998-07-04

Degree:Ph.D

Type:Dissertation

University:The Medical College of Wisconsin

Candidate:Potter, David

Full Text:PDF

GTID:1461390014976191

Subject:Biology

Abstract/Summary:

PDF Full Text Request

A pET-11a plasmid encoding the cDNA for rat mevalonate kinase was utilized for expression of recombinant enzyme in E. Coli. The enzyme has a specific activity of 30 U/mg, and apparent Michaelis constants (K;The cDNA encoding human mevalonate kinase has also been over expressed and the recombinant enzyme isolated. The human enzyme exhibits a V...

Keywords/Search Tags:

Mevalonate, Enzyme

PDF Full Text Request

Related items

1	Isobutene formation from 3-hydroxy-3-methylbutyrate (3-HMB) by the Saccharomyces cerevisiae diphosphomevalonate decarboxylase (ScMDD) and directed enzyme evolution to improve enzyme function
2	Optimization Of Fermentation Process Of Methylobacterium Extorquens AM1 For Efficient Mevalonate Production
3	Dynamic Flux Regulation Of Artificial Carbon-saving EP-bifido Pathway Improves Mevalonate Production In E.coli
4	Construction And Optimization Of The Mevalonate Pathway In Escherichia Coli For Production Of Isoprene
5	Study On High Cell Density Fermentation For Production Of Mevalonate By Methylobacterium Extorquens
6	Engineering a heterologous mevalonate pathway for the production of isoprenoids in Escherichia coli
7	Engineering A Mevalonate Pathway In Halomonas Bluephagenesis For The Production Of Lycopene
8	Development Of Enzyme-catalyzed Biosensors And Their Analytical Applications
9	The Preparation Of Enzyme On The Research Of Compounded Enzyme For Food Industry
10	The Construction Of Several Novel Enzyme Biosensors And Their Applications In Biosensing