The modelling and analysis of the dynamic performance of perfusion chromatography in fixed-bed and periodic countercurrent column operations and parameter estimation and model discrimination using experimental data

Comparisons of dynamic simulation results for single and multi-component adsorption in columns operated in fixed bed and periodic countercurrent mode show that the periodic countercurrent operating mode nearly always gives superior performance. Furthermore, the performance of columns operated in the periodic countercurrent mode can be independent of the values of parameters that determine the mass transfer resistances of the system.; Simulation results over a range of values of several adsorbent particle structural and adsorption mechanism parameters show that intraparticle fluid flow in perfusive particles can improve adsorption system performance if it is of sufficient magnitude. However, performance with purely diffusive particles can be better than with perfusive particles, if purely diffusive particles can be made with higher values of C{dollar}rmsb{lcub}T{rcub}{dollar}, the maximum equilibrium concentration of adsorbate in the adsorbed phase of the adsorbent particle, than otherwise comparable perfusive particles.; A proposed method for parameter estimation in the Laplace transform domain is able to estimate intraparticle mass transfer parameters in linear adsorption models from simulated experimental data. The dynamic nonlinear model for purely diffusive particles having a monodisperse porous structure describes most appropriately experimental breakthrough data for the adsorption of bovine serum albumin onto porous anion-exchange particles packed in a column.