| In the vision of performing analysis at the site of interest has increased the desire for a Laboratory on a Chip. Miniaturization of instrumentation such as mass spectrometers, NMR probes, and separation systems, has thus been the focus of many researchers in analytical chemistry. Also, making these devices cheaply such that they are disposable has spurred on interest of plastics as substrates. The work presented here focused on the development and applications of capillary electrochromatographic separation systems fabricated in Poly(dimethyl siloxane) (PDMS).; Collocated monolithic support structures (COMOSS) were fabricated in a highly expensive quartz substrate which prevented disposing of these devices. While highly efficient analysis was performed, fouling and clogging of channels required the discovery of cheaper plastic alternatives that are presented. New stationary phases were proposed. Channel geometry of the COMOSS separation systems, and injection techniques into the separation column were analyzed and improved upon. Finally, real world analysis, such as proteomic analysis, were demonstrated to show applicability and usefulness of these PDMS COMOSS separation devices. |
