Aggregation and proteolytic degradation can significantly decrease the recovery yields of recombinant proteins overproduced in the gram-negative bacterium Escherichia coli. Expression at low growth temperatures holds great promise in alleviating these problems since proteolysis is reduced and proper folding is facilitated under these conditions. Unfortunately, the transcriptional efficiency of traditional promoters decreases with the cell growth temperature. When E. coli is transferred from a physiological temperature of 37... |