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Statistical aspects of using genetic markers for individual identification in capture-recapture studies

Posted on:2006-04-03Degree:Ph.DType:Dissertation
University:Colorado State UniversityCandidate:Lukacs, Paul MFull Text:PDF
GTID:1458390008454354Subject:Agriculture
Abstract/Summary:
The use of an animal's genotype as a mark for capture-recapture studies has become increasingly common in wildlife research. Frequently, animals are sampled in a non-invasive way such as collecting hair from rubs, shed feathers or feces. DNA is extracted from the samples and genotyped at a set of microsatellite loci to identify individuals. In this case, the animal's mark is self assigned. This leads to problems with misidentification of individuals. In addition, samples are often passively collected which causes a break down in the standard capture-recapture assumption of instantaneous sampling.; This dissertation focuses on developing new ways of handling the unique circumstances associated with DNA-based capture-recapture studies. I develop a method to account for genotyping error in closed population estimates of abundance. This method is used to extent the standard likelihood-based closed population capture-recapture models, the finite mixture models and the conditional likelihood models. I extend the robust design model to properly estimate survival and abundance in the face of genotyping error and allow multiple sources of data to be brought together. A new method is developed that uses additional information in dung surveys generated from multiple detections of an individual within a sampling occasion to better estimate abundance.; I present suggestions for applying DNA-based capture-recapture sampling for Canada lynx (Lynx canadensis) in Colorado. Sampling feces in summer appears to be the most effective way to sample the lynx population. Feces are easier to collect in the field than hair and are more amenable to DNA extraction.; Finally, I present a review of the literature applicable to DNA-based capture-recapture studies. The focus of the review is to provide a starting point for researchers looking to broaden the scope of their DNA-based capture-recapture study beyond simple estimates of abundance.
Keywords/Search Tags:Capture-recapture, Abundance
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