Studies of physical phenomena and optimization of DNA sequencing performance in capillary electrophoresis

The goal of this Dissertation is to demonstrate a possibility of controlling and improving quality and efficiency of the capillary electrophoretic separation by using a number of the novel techniques for injection and detection of fluorescence labeled DNA fragments. The proposed techniques include optimization of the sample run conditions by solving a contact problem for buffer/polymer interface, a field-flow approach to DNA sample detection in multi-capillary arrays, the optimized electrokinetic injection of DNA sample into the specially designed capillary and thermally-controlled Polymerase Chain Reaction (PCR) chip prototype.; In order to solve the problems mentioned above, we used computer simulations for obtaining electric and thermal fields distribution and analyzed a propagation of DNA fragments through areas of the non-uniform fields. We found a fairly good agreement between our simulations and experimental results obtained in DNA Sequencing Lab.