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Droplet-Interface Bilayer Technologies for Membrane Protein Analysis and Molecular Trafficking Measurements

Posted on:2014-02-14Degree:Ph.DType:Dissertation
University:University of Massachusetts AmherstCandidate:Lein, Max JFull Text:PDF
GTID:1451390008456417Subject:Chemistry
Abstract/Summary:
Droplet-interface bilayers (DIBs) have been developed as a miniature model membrane system with distinct advantages over cell-based and other artificial membrane systems. This dissertation will describe a number of projects relating to DIB research, including: (i) the creation of new devices that allow the repeated addition and mixing of reagents to one side of a droplet-based membrane system; (ii) measurements of peptide and polymer mediated protein translocation across lipid bilayers; (iii) efforts to synthesize eukaryotic ion channels in DIBs; (iv) investigations on the asymmetric lipid dependence of the potassium channel KirBac1.1.
Keywords/Search Tags:Membrane
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