Dynamic contrast-enhanced magnetic resonance imaging & fluorescence microscopy of tumor microvascular permeability | Posted on:2009-04-19 | Degree:Ph.D | Type:Dissertation | University:The University of Arizona | Candidate:Jennings, Dominique Louise | Full Text:PDF | GTID:1444390005459717 | Subject:Engineering | Abstract/Summary: | | Microvascular permeability is a pharmacologic indicator of tumor response to therapy, and it is expected that this biomarker will evolve into a clinical surrogate endpoint and be integrated into protocols for determining patient response to antiangiogenic or antivascular therapies. The goal of this research is to develop a method by which microvascular permeability (Ktrans) and vascular volume (nu p) as measured by DCE-MRI were directly compared to the same parameters measured by intravital fluorescence microscopy in an MRI-compatible window chamber model. Dynamic contrast enhanced-MRI (DCE-MRI) is a non-invasive, clinically useful imaging approach that has been used extensively to measure active changes in tumor microvascular hemodynamics. However, uncertainties exist in DCE-MRI as it does not interrogate the contrast reagent (CR) itself, but the effect of the CR on tissue water relaxivity. Thus, direct comparison of DCE-MRI with a more quantitative measure would help better define the derived parameters. The combined imaging system was able to obtain both dynamic contrast-enhanced MRI data high spatio-termporal resolution fluorescence data following injection of fluorescent and gadolinium co-labeled albumin. This approach allowed for the cross-validation of vascular permeability data, in relation tumor growth, angiogenesis and response to therapy in both imaging systems. | Keywords/Search Tags: | Tumor, Permeability, Imaging, Microvascular, Response, Dynamic, Fluorescence, DCE-MRI | | Related items |
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