| Certain premalignant conditions of gastric cancer are related to particular genetic alterations. Polymorphisms of the IL-8, TNF-alpha, and p53 genes -- which play a significant role -- can be identified in patients suffering from gastritis and IM. H. pylori infection is another important factor, which influences the effects of the investigated genes and polymorphisms.;For a better understanding of the role of polymorphisms or mutations in the etiology of gastric disease, laser-microdissection of the affected cells, identified by IHC, is proposed. This could afford early recognition of patients at risk of developing micro- or macroscopic, pathological lesions as well as the introduction of preventive measures.;With the application of high-capacity screening methods such as resequencing arrays also offering the additional advantage of an expedited diagnosis, further genetic factors can be identified. Various methods for polymorphism detection are available for identifying sequence alterations. Unfortunately, the GeneChip p53 Probe Array is apparently not reliable enough for use as a stand-alone DNA resequencing method. Based on my research results certain types of resequencing microarray may prove a sensitive and reliable method for genetic screening for diagnostic purposes. Nevertheless, CS is always recommended for verification. |
