| To facilitate rapid detection of potential bioterrorist agents, development of an inexpensive, adequately sensitive and specific, user-friendly, robust, and rapid field-based biosensor is advantageous for preventing the spreading of infections. In this study, fabrication and performance analysis of an indium tin oxide (ITO)-polyaniline (Pani) biosensor are highlighted. The biosensor design is based upon the specific nature of antibodies and electroactive properties of Pant The ITO-Pani biosensor is comprised of two components: immunosensor and amperometric measuring device. The immunosensor is made of a conductive ITO substrate coated with Pani. The conductive substrate is functionalized into a biosensor by immobilizing antibodies within the polymer matrix. Experimental conditions such as the Pani size, antibody concentration, immobilization method, and incubation time were assessed to find the best condition for the biosensor fabrication. Using bovine viral diarrhea virus as a model pathogen, the sensitivity of the biosensor was 104 cell culture infective dose per ml (CCID/ml) with a linear range up to 106 CCID/ml of virus concentration. |
