| Mitosis is a highly dynamic process by which the duplicated genome is segregated equally into a mother and daughter cell. Mitosis requires the coordination and regulation of numerous protein activities in order to accurately segregate the genome. The complexity of mitosis has made the simple budding yeast, Saccharomyces cerevisiae, an attractive system to study mitosis. This dissertation focuses on the identification of proteins with mitotic functions and the regulation of mitosis by the Spindle Assembly Checkpoint. I describe the differential regulation of APCCdc20 substrates during mitosis by the Spindle Assembly Checkpoint. I also identify novel genes with roles in mitosis and do an in depth analysis of one of these genes, IRC15. I show that Irc15 is a microtubule associated protein required for proper tension between kinetochores and accurate segregation of chromosomes in mitosis. |
