| Purpose: The primary goal of the present study was to design Doxorubicin(DOX)-loaded superparamagnetic iron oxide(SPIO)nanoparticles(NPs)coated with mesenchymal stem cell(MSC)membranes,and explore their effect on colon cancer in vitro and in vivo.Methods: DOX-SPIO were coated with MSC membranes using an extruder,and the morphological characteristics of MSC membrane-camouflaged nanodrug(DOX-SPIO@MSCs)evaluated by transmission electron microscopy(TEM)and NP-tracking analysis.Drug loading and pH response were assessed by UV spectrophotometry.Intracellular colocalization was analyzed using NP-treated MC38 cells stained with 3,3′-dioctadecyloxacarbocyanine perchlorate and Hoechst 33342.Cellular uptake was analyzed using an inverted fluorescence microscope and flow cytometry and cytotoxicity evaluated by Cell counting kit-8 assay.Biological compatibility was assessed by hemolysis analysis,immunoactivation test and leukocyte uptake experiments.Furthermore,intravenous injection of chemotherapy drugs into MC38 tumor-bearing C57BL/6 mice was used to study anti-tumor effects.Results: Typical core-shell NP structures were observed by TEM.Particle size remained stable in fetal bovine serum and phosphate buffered saline(PBS).Compared with DOX-SPIO,DOX-SPIO@MSCs improved cellular uptake efficiency,enhanced anti-tumor effects,and reduced the immune system response.Animal experiments demonstrated that DOX-SPIO@MSCs enhanced tumor treatment efficacy while reduc-ing systemic side effects.Conclusion: Our experimental results demonstrate that DOX-SPIO@ MSCs are?a promising targeted nanocarrier for application in trea-tment of colon cancer. |
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