Study On The Role And Mechanism Of Livin In TGF-?1 Induced EMT In Human Renal Tubular Epithelial Cell

Objective: Renal interstitial fibrosis is accepted as a crucial character in the progress of chronic kidney diseases(CKD),especially to the end stage renal failure(ESRF).Meanwhile,epithelial-mesenchymal transition(EMT)plays a significant role in renal interstitial fibrosis and transforming growth factor beta(TGF-?1)is considered as one of the most important fibrotic factors.Livin and lncRNA-ATB are key regulators of EMT in kinds of tumors,which might also be involved in human renal tubular EMT.In this study,we detect the expression of Livin in human renal tubular EMT and study the potential mechanism of Livin and lncRNA-ATB in TGF-?1 induced human renal tubular EMT.Methods: In vivo,unilateral ureteral occlusion model(UUO model)was built in male rats for 14 days.Test the level of Scr and BUN in blood to ensure that UUO model was built.The E-cadherin,?-smooth muscle actin(?-SMA),vimentin and Livin expression were observed by immunofluorescent staining.In vitro,HK2 cells were treated by TGF-?1 to induce EMT,then we use quantitative PCR,western blot analysis,immunofluorescence and transwell techniques to verify the relation between Livin and EMT.Furthermore,Livin was knocked down to investigate the function that Livin played in EMT.In the third part,we detected the level of lncRNA-ATB and tried to find the relationship between Livin and lncRNAATB in human renal EMT.Results:UUO model successfully induced renal fibrosis in vivo.Compared to those in control and sham groups,E-cadherin was down-expressed and ?-SMA,vimentin and Livin were highly-experssed in UUO group.qPCR also proved those results.Livin was up-regulated in TGF-?1 treated HK2 cells.Livin and lncRNA-ATB were all increasingly expressed during the process of EMT.The silence of Livin could downregulate lncRNA-ATB and alleviate EMT.Furthermore,the silence of lncRNA-ATB could lessen the severity of TGF-?1 induced EMT.LncRNA-ATB could aggravate TGF-?1 induced EMT without influence the expression of Livin.Conclusion: EMT is involved in the process of RIF and Livin participates in the regulation in RIF.Livin promotes TGF-?1-induced human renal tubular epithelialmesenchymal transition through lncRNA-ATB.The silence of Livin might be an effect targeted therapy gene in renal fibrosis.