| Objective:Polycystic ovarian syndrome(PCOS)is a frequent reproductive endocrine and metabolic disease that affects menstruation,fertility,quality of life and long-term health of patients.Gut is called the second brain and gut microbiota can modulate the physiological and pathological activities of the body through nerve,endocrine,and immunity.Gut microbiota plays a critical role in the pathogenesis of metabolic diseases and inflammation.The effect of gut dysbiosis on the pathogenesis of PCOS aroused extensive attention among researchers.The study aims to verify the correlation between PCOS and microbiota in the gut or in the oral cavity,and sheds light on the influence of dysbiosis of gut microbiota on the pathogenesis of PCOS through treating germ-free mice with fecal microbiota transplantation(FMT).Methods:1.The study collected feces and saliva from 20 healthy volunteers and 20 PCOS patients who visited the department of gynecology endocrinology at Peking Union Medical College Hospital(PUMCH)from February 2019 to March 2019,and analyzed by 16s ribosome DNA sequencing to identify the differences in gut microbiota and oral microbiota between PCOS patients and healthy people.2.The study chose eight-week-old C3H sterile mice divided into two groups randomly,and treated mice with fecal microbiota transplantation(FMT)from PCOS patients or healthy people to analyze the difference in the hallmarks of PCOS and gut microbiota between two groups.Results:THE FIRST PART the contrast between PCOS patients and healthy controls 1.Gut microbiota:Compared with the healthy control group,a diversity indexes of the gut microbiota in PCOS group were lower,however,without statistical difference,and PcoA and ANOVA did not show ? diversity difference between two groups.The relative abundance of Eggerthella was higher in PCOS patients compared with control women,and it was positively correlated with obesity index and insulin resistance.The relative abundance of butyrate-producing Lachnobacterium,Peptococcaceae and rc4-4 was lower in PCOS patients compared with control group,and they showed negative correlattion with obesity index and insulin resistance.2.Oral flora:Compared with control women,the a diversity of the PCOS oral microbiota increased(P<0.05),however,without difference in ? diversity.The PCOS group more frequently had periodontitis-related or gingivitis-related Fusobacteriaceae,Fusobacterium,Spirochaetaceae,Treponema,Parvimonas,and Capnocytophaga compared with control women.THE SECOND PART the sterile mouse experiment 1.The PCOS-FMT mouse model had higher food intake,obesity index,blood lipid level and prolonged diestrus,and the ability of convert proestrus to estrus was weaker compared with control-FMT mouse.2.Two FMT groups had ? diversity difference(P<0.05),without difference in a diversity.Compared with control-FMT mouse,the relative abundance of the lipopolysaccharide-producing Bilophila was higher in the PCOS-FMT group and Bilophila had positive correlation with triglyceride level.The relative abundance of butyrate-producing Eubacterium was lower in PCOS-FMT mice compared with control-FMT mouse,and they showed negative correlattion with food intake,body weight,body fat and insulin resistance.Conclusions:1.The study showed remarkable differences in gut microbiota between PCOS patients and healthy people and the discrepant gut microbiota had positive or negtive correlation with hallmarks of PCOS disease,which demonstrated the correlation between PCOS and gut microbiota.There were also some differences in oral microbiota between PCOS patients and healthy people and the study showed PCOS may have correlation with oral inflammatory disease.2.The experiment of sterile mouse with gut microbiota colonization of demonstrated that gut microbiota dysbiosis can be a contributing factor in the pathogenesis of PCOS,acting on obesity,lipid metabolism and menstrual cycle of PCOS,which may attribute to the increased lipopolysaccharide-producing bacteria of and decreased butyrate-producing bacteria. |
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