Effects Of Ximeining On Glucose And Lipid Metabolism And Liver AMPK/ACC Signaling Pathway In Type 2 Diabetic Model Rats

Objective:A rat model of type 2 diabetes insulin resistance was duplicated,the effect of Ximeining on glucose and lipid metabolism and liver AMPK/ACC signaling pathway in rats were observed.Objective to explore the mechanism of Ximeining in improving metabolic disorders and insulin resistance.Methods:60 male SD rats were randomly divided into two groups,of which 10 were used as blank groups,the remaining 50 rats were replicated with type 2 diabetes insulin resistance rat model by using fat emulsion combined with low-dose intraperitoneal injection of STZ for 2 times,rats with fasting blood glucose exceeding 16.7mmol/L were used as animal models for modeling.After that,the rats were randomly divided into model group,Ximeining group and metformin group.The blank group and the model group were fed distilled water,the Ximeining group was given Ximeining extract,and the metformin group was fed with Metformin Hydrochloride Tablets suspension,the rats in each group were administrated to the stomach for 30 days.During this period,fasting blood glucose in each group was regularly detected by tail blood sampling.After the experiment,the levels of HbA1c,TC,TG,HDL-C and LDL-C in the serum of each group were analyzed by biochemical analyzer,radioimmunoassay was used to analyze the serum levels of FINS in each group,and the insulin resistance index(HOMA-IR)of each group was calculated.The content of adipocytokine APN,Resistin and visfatin in serum of each group was detected by ELISA method,the pathological changes of liver tissues in each group were observed by HE staining.The expression of AMPKα mRNA and ACC mRNA in liver tissues of each group was detected by real-time fluorescence quantitative PCR,and the expression of AMPKa,p-AMPKα,ACC,p-ACC protein in liver tissues of each group was detected by Western Blot.Results:1.Blood glucose and insulin related indexes:Compared with the model group,the levels of FBG,HbAlc,FINS and HOMA-IR decreased in the Ximeining group,and the difference was statistically significant(P<0.05).2.Blood lipid index:the level of TC,TG and LDL-C in the serum of Ximeining group decreased,and the level of HDL-C in the serum increased,which was statistically significant compared with the model group(P<0.05).3.Adipocyte Factor:Ximeining can reduce the content of Resistin and visfatin in the serum of T2DM model rats,and increase the content of APN in the serum,and the difference is obvious compared with the model group(P<0.05).4.Pathological section of liver HE staining:Ximeining can reduce the state of liver cell arrangement disorder and fatty degeneration in T2DM model rats,which is significantly different from that of the model group.5.AMPK/ACC signaling pathway in the liver:Compared with the model group,Ximeining can significantly increase the expression of AMPKa mRNA,protein and p-AMPKa protein in the liver tissue of T2DM model rats,and up regulate the expression of p-ACC protein in the rat liver tissues,and the difference was statistically significant(P<0.05).Conclusion:1.Ximeining can correct the disorder of glucose and lipid metabolism in T2DM rats,it has the effect of reducing sugar and reducing fat.2.Ximeining can reduce the insulin level of T2DM model rats,improve the sensitivity of islet β cells,and also reduce the level of HOMA-IR and improve insulin resistance.3.Ximeining can reduce the insulin resisitance of T2DM model rats by regulating the contents of adipocytokine APN,Resistin and visfatin.4.Ximeining can protect the liver of T2DM model rats and alleviate their pathological changes.5.Ximeining affects the AMPK/ACC signaling pathway in the liver of T2DM rats,which may be one of the mechanisms of improving the metabolic disturbance and insulin resistance.