The Role Of TBK1 In Head And Neck Squamous Cell Carcinoma

Background:Head and neck tumor accounts for about 10%of all malignant tumors,and its morbidity is about the sixth of all malignant tumors.Statistics show that more than 90%of head and neck cancers are head and neck squamous cell carcinoma.Nowadays,there are many studies on squamous cell carcinoma tumor markers,including P63,CK5/6,P40,and so on.However,whether at home or abroad,the studies and clinical applications of these tumor markers in HNSCC are very few.Hence,it is very meaningful and necessary to study the specific tumor markers and targeted therapy of HNSCC.Methods:Firstly,by using GEO database the associated sub-datasets were selected and the expression of TBK1 in HNSCC was preliminarily analyzed.Secondly,using tissue microarray as clinical tissue samples,immunohistochemical staining of TBK1 protein expression in HNSCC tumor tissues was performed.Thirdly,the protein expression of TBK1 was inhibited in FaDu cells and Cal-27 cells.Lastly,the internal mechanism was explored through CCK-8,flow cytometry and Western blot.Results:The results of GEO database showed that TBK1 protein expression was significantly higher in HNSCC tumor tissues than in normal tissues.Immunohistochemical results of tissue microarrays were consistent with the results of the GEO database analysis.After inhibiting the expression of TBKl protein,the results of CCK-8 showed that the proliferation ability of FaDu cell and Cal-27 cell was significantly decreased;the results of flow cytometry showed that the apoptosis of FaDu cell and Cal-27 cell was significantly increased;the results of Western blot showed that the expression of p65,Livin and Survivin proteins in FaDu cell and Cal-27 cell was significantly changed.Conclusions:(1)The present study revealed that TBK1 protein expression in HNSCC tumor tissue was higher than that in normal tissue;(2)When TBK1 protein expression was inhibited in HNSCC tumor cells,the proliferation ability was significantly decreased and the apoptosis was significantly increased,which may be related to the apoptosis inhibition process involved in the Livin protein and Survivin protein.