| Objective:1.This study was to establish a model of migraine-epilepsy comorbidity,and to observe its behavioral manifestations.2.The changes of c-Fos expression in Sp5C,HSP70 and BDNF expression in the CA3 area of hippocampus were detected by immunohistochemistry to explore the establishment of the model of migraine-epilepsy comorbidity.3.The changes of CGRP concentration and BDNF concentration in the plasma were measured by enzyme-linked immunosorbent assay(ELISA).The expression of ASIC la,ASIC3 and CACNA1A was detected by qPCR and Western Blot to explore the mechanism of the migraine-epilepsy comorbidity.Methods:1.The corresponding chemical substances were injected on dura mater through cannula to establish migraine model.The lithium chloride-pilocarpine was injected intraperitoneally to establish epilepsy model.The rats were randomly divided into control group,migraine group,seizure group and comorbid group.2.The scratching and the pain sensitivity were tested among the control group,migraine group and comorbid group.and the corresponding chemical substances were injected on dura mater.The latent period and the Racine scale were tested among the control group,seizur group and comorbid group.3.The changes of CGRP and BDNF in the plasma were compared among different groups by ELISA.The expression of c-Fos protein,HSP70 and BDNF in different groups were compared by immunohistochemistry.The expression of ASIC la,ASIC3 and CACNA1A was detected by qPCR and Western Blot.Results:1.The comorbidity group compared with the migraine group,the number of scratches and the pain sensitivity were increased significantly.Compared with the seizure group,the comorbidity group had a significant decrease in latency,and the proportion of seizure rats that reached 6 scale was higher.2.The expression of CGRP in the plasma between the migraine group and the comorbidity group was not significantly different.The expression of BDNF in the plasma between the seizure group and the comorbidity group was not significantly different.3.The expression of c-Fos in the trigeminal caudate nucleus in the comorbidity group was significantly higher compared with the migraine group.Compared with the seizure group,the expression of HSP70 in the C3 region of hippocampus was not significantly increased in the comorbidity group,but the expression of BDNF in the CA3 region was significantly increased in the comorbidity group.Conclusions:1.The stimulation of the superior sagittal sinus by chemical substances could lead to the increase of the number of scratching and pain sensitivity,and the increase of c-Fos expression in Sp5C region,suggesting that the migraine model is established successfully.The rats in the seizure group reached 4 scale or above,suggesting that the epilepsy model is established successfully.2.The comorbidity model was established successfully because the increasing number of scratching,the increasing pain sensitivity,the decreasing latent period.3.The expression of c-Fos in the comorbidity group was higher than that in the migraine group,suggesting that epilepsy may aggravate the onset of migraine,which also explains why migraine patients with epilepsy have more headache attacks.4.The expression of BDNF in the C3 region of the hippocampus in the comorbidity group was higher than that of the epilepsy group,suggesting that migraine may aggravate epilepsy,which also explains why epilepsy patients with migraine have much more serious attacks. |
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