Identification Of PLC Gene Family In Wheat,Barley And Brachypodium Distachyon And Functional Analysis Of TaPI-PLC1-2B

Phospholipase C(PLC)is a member of the phospholipase family and plays an important role in regulating plant growth,development and response to stress.Systematic analysis has been carried out in Arabidopsis,maize,rice and other plants.However,the PLC genes in wheat have not been systematically analyzed before.In this study,we identified the PLC gene family of wheat,barley and Brachypodium distachyon,including sequence characteristics,conserved domains,chromosomal locations,phylogenetic relationships,cis-acting elements,tissue specific expression levels,and expression patterns under abiotic stresses such as low temperature,drought,and salt stress.The biological function of TaPI-PLC1-2B gene was further analyzed by transgenic materials of rice and Arabidopsis.The main results are as follows:1.The TaPLC family genes of wheat were analyzed genome-wide.A total of 26 TaPLC genes were identified in the wheat genome,which were divided into two subfamilies,namely,TaPI-PLC and TaNPC,according to the different domains carried.PI-PLC and NPC gene sequences were highly conserved and significantly different in wheat.In addition,the type and number of cis-regulatory elements in the respective promoters of the TaPLC gene are different,which is consistent with their unique expression patterns under specific tissue and different abiotic stresses.By analyzing the expression patterns of some TaPLC gene family members under salt stress,drought stress and low temperature stress,it was found that TaPLC family members were responsive to all three abiotic stresses,especially more sensitive to salt stress.2.The whole genome analysis of PLC family of Brachypodium distachyon and barley was carried out,8 Bd PLC genes and 11 Hv PLC genes were confirmed,and they were divided into Bd PI-PLC(Bd PI-PLC1-4),Bd NPC(BDNPC1-4),Hv PI-PLC(Hv PI-PLC1-5)and Hv PI-PLC(Hv NPC1-6).The gene structures,phylogenetic relationships,chromosomal positions,cis-acting elements and expression patterns related to abiotic stress were analyzed.The expression profile under drought,low temperature and salt stress showed that PLC genes of some barley and Brachypodium distachyon were significantly up-regulated,indicating that they played an important role in response to low temperature,high temperature,drought and salt stress.3.It was found that the overexpression of TaPI-PLC1-2B gene was significantly changed in arabidopsis and rice,which improved the drought resistance and salt tolerance of transgenic rice and Arabidopsis.Transcriptome analysis of transgenic rice also found that the over-expression of the TaPI-PLC1-2B gene in the signal transduction pathways related to drought and salt stress in rice mainly involved phosphoinositide signaling system,plant hormone signal transduction and MAPK signal pathway.4.The TaPI-PLC1-2B gene promoter sequences upstream to cloning,analysis of the cis component,and carries on the 5 'end missing analysis,identify TaPI-PLC1-2B gene promoter activity has started and organize express specificity,main expression in the root,leaf blade not seen its expression activity,speculated that its downstream genes TaPI-PLC1-2B genes may on wheat growth and development of specific areas at a specific time plays an important role.