Evaluation Of Protein PeBL1 From Brevibacillus Laterosporus To Induce Resistance Against Myzus Persicae On Tomato And Cucumber

Green peach aphid(Myzus persicae),a major pest in tomato and cucumber,is typically managed by the use of chemical pesticides.In the present study,the potential of a protein elicitor(Pe BL1)of Brevibacillus laterosporus,a broad-spectrum elicitor evaluated from B.laterosporus strain A60 a bacterial entomopathogen,to induce a defense response in tomato and cucumber against M.persicae was studied.Biocontrol potential in B.laterosporus is not only limited to insects,nematodes,and mollusks but also encompasses phytopathogenic fungi and bacteria.Biocontrol potential in entomopathogenic species has been analyzed on insects in the orders Coleoptera,Lepidoptera,and Diptera.Four different concentrations of Pe BL1 solution,i.e.,(88.72,53.23,26.61,and 22.17)μg m L-1 along with positive(water)and negative 88.72 μg m L-1(buffer 50 m M Tris-HCl,p H 8.0)controls,for tomato and(70.58,42.34,21.17 and 17.64)μg m L-1 along with positive(water)and negative 70.58 μg m L-1(buffer 50 m M Tris-HCl,p H 8.0)controls,for cucumber were applied on the seeds and seedlings of tomato and cucumber.Population growth rates of M.persicae(second and third generation)decreased with Pe BL1 treatments as compared with controls.In a host selection assay,M.persicae showed preference for colonizing control plants as compared to tomato and cucumber seedlings treated with Pe BL1.Tomato and cucumber leaves treated with Pe BL1 gave rise to a hazardous surface environment for M.persicae due to formation of trichomes and wax.Jasmonic acid(JA),salicylic acid(SA),and ethylene(ET)showed significant accumulation in tomato and cucumber seedlings treated by Pe BL1.The following results showed that Pe BL1 significantly modified the tomato and cucumber leaf surface structure to reduce reproduction and deter colonization by M.persicae.Defense processes also included activation of JA,SA,and ET pathways.The study provides evidence for use of Pe BL1 in the protection of tomato and cucumber from M.persicae.