The Mechanism Of The Application Risk Of Lipotropic Polysaccharides From Lactobacillus Rhamnosus GG And L.Casei BL23

Natural polysaccharides have received much attention to ameliorate hepatic steatosis induced by high fat diet(HFD).Here,we tested the anti-hepatosteatosis effect of the exopolysaccharides(EPS)from two well-known Lactobacillus strains Lactobacillus rhamnosus GG(LGG)and L.casei BL23 in zebrafish model.While both EPS reduced hepatic steatosis in zebrafish fed HFD,the BL23 EPS,but not LGG EPS,induced liver inflammation and injury.This is due to that BL23 EPS induced gut microbial dysbiosis characterized by an expansion of endotoxin-bearing Proteobacteria,while LGG EPS promoted microbial homeostasis,The mechanism underlying the opposite differentiation of LGG EPS-and BL23 EPS-associated microbiotas lies on that LGG EPS,but not BL23 EPS,can directly activate the intestinal HIF1?,and the increased HIF1? boosts local antimicrobial peptides(AMP)to facilitate the microbial homeostasis.Together,our data indicated that anti-hepatic steatosis polysaccharides should be applied with caution,and highlighted the importance of HIF1?-AMP axis in counteracting adverse aspects of polysaccharides fermentation.