Chemiluminescence/Electroluminescence Biosensor Based On Microfluidic Chip

With the highly increasing demand of biological analysis,the high-throughput and automated batch analysis technologies and methods have attracted more and more attention from people.The microfluidic technology perfectly meets the above requirements.Microfluidic technology has the advantages of high throughput,low consumption,portable,automatic and easy integration,and can be well combined with various analytical and detection methods.Therefore,it has always been a hot topic of research since the beginning in the 1990 s.Although microfluidic technology is according with human conception for bioanalysis technology,its low consumption of reagents requires high sensitivity of the corresponding analytical detection technology.Chemiluminescence and electrochemiluminescence are both commonly used in analytical techniques and are highly sensitive with no background interference in action.They can quickly,easily,and efficiently achieve signal acquisition and detection of substances.Therefore,this thesis is for specific and highly sensitive chemiluminescence/electrochemiluminescence biosensor established on microfluidic chip.First of all,we briefly summarized the development and advantages of microfluidic chips,and then introduced the usually methods for manufacturing and surface modification in detail.Finally,we presented an overview of different types of microfluidic chip-based biosensors recently to comprehend its development,advantages and research significance.Second,the specific binding of aptamer was used to capture and identify thrombin protein on a microfluidic multi-channel array chip.The gold nanoparticles were functionalized with abundant DNAenzymes by rolling amplification amplification,which can enhance the chemiluminescence of luminol and hydrogen peroxide,for achieving double signal amplification and highly sensitive detection of thrombin.Third,a discontinuous microfluidic bipolar-electrode platform was constructed,and a large number of DNA-templated silver nanoclusters were generated on the gap by rolling amplification amplification.The electrochemiluminescence signal was recovered for the strong conductivity of silver nanoclusters.The ATP detecting was easily achieved by combining the ATP aptamer capturing with signal recovery in this process.Finally,electrochemiluminescence detection of cell numbers was performed on the discontinuous microfluidic bipolar-electrode platform again.This method utilized the strong conductivity of ions contained cell lysate by deionized water and sonication.As a result,electrochemiluminescence signal was recovered after lysate was added to gap channel.