| ?. Effects of a novel 3D zinc coordination polymer (ZnCP) on the proliferation of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cellsObjective:To prepare a novel 3D ZnCP and investigate its effects on the proliferation of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells.Methods:1. Zn(NO3)2·6H2O, bpydbH2, Htz(triazole) and DMF were heated in a water bath, and then allowed to cool and precipitate before eventually forming the novel 3D ZnCP; 2. Passage human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells were randomized into the blank control group, DMSO vehicle group, positive control group (adriamycin), 10?mol/L ZnCP group,20?mol/L ZnCP group, and 40umol/L ZnCP group. Each group was separately incubated for 12h,24h, and 48h before using MTT assay to detect the inhibitory effect of the novel 3D ZnCP on the proliferation of these two in vitro-cultured cells.Results:1.The OD values of human osteosarcoma MG-63 cells incubated for 12h,24h and 48h were 1.308±0.0645,1.193±0.0214, and 1.095±0.0215, respectively, in the blank control group;1.207±0.0103,1.084±0.022, and 0.981±0.048, respectively, in the vehicle (DMSO) group; and 1.147±0.003,0.947±0.0036, and 0.732±0.0031, respectively, in the positive control (adriamycin) group; the OD values at 12h following treatment with 3 concentrations of ZnCP were 1.179±0.0122,1.112±0.0111, and 0.93±0.0182, respectively; the OD values at 24h following treatment with 3 concentrations of ZnCP were 0.966±0.0072,0.859±0.0075, and 0.488±0.0119, respectively; and the OD values at 48h following treatment with 3 concentrations of ZnCP were 0.766±0.0053,0.644±0.008, and 0.211±0.0026, respectively.2.The OD values of human osteosarcoma U-2 OS cells incubated for 12h,24h and 48h were 1.290±0.0136,1.200±0.0046, and 1.148±0.0255, respectively, in the blank control group; 1.193±0.0145,1.082±0.0274, and 1.007±0.0223, respectively, in the vehicle (DMSO) group; and 1.121±0.0046,0.953±0.0036, and 0.789±0.0021, respectively, in the positive control (adriamycin) group; the OD values at 12h following treatment with 3 concentrations of ZnCP were 1.167±0.0162,1.07±0.031, and 0.835±0.0627, respectively; the OD values at 24h following treatment with 3 concentrations of ZnCP were 0.993±0.0127,0.908±0.0235, and 0.468±0.0599, respectively; and the OD values at 48h following treatment with 3 concentrations of ZnCP were 0.831±0.0165, 0.729±0.0076, and 0.266±0.0081, respectively.3.With regard to the inhibitory effect on the proliferation of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells, there were statistically significant differences between the blank control group and the vehicle (DMSO) group in OD values (P<0.05). Compared to the vehicle group, there was no statistically significant difference in OD value at 12h following treatment with 10?umol/L ZnCP (P>0.05), yet statistically significant differences in OD value at 12h following treatment with both 20?mol/L ZnCP and 40?mol/L ZnCP (P<0.05). There were statistically significant differences in OD value across the 3 concentrations, as well as across the groups, at 24h and 48h following treatment (P<0.05).Conclusion:DMSO had cytotoxic effect on both human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells. ZnCP had inhibitory effect on the proliferation of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells, which presented significant concentration and time dependence.?. The mechanism of a novel 3D ZnCP inhibiting activity of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cellsObjective:To explore the effect of a novel 3D ZnCP on the inhibiting activity of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells and its mechanism of actionMethods:Passage human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells were randomized into the blank control group, 10?mol/L ZnCP(24h) group, 20umol/L ZnCP(24h) group,40?mol/L ZnCP(24h) group,40?mol/L ZnCP(12h) group, and 40?mol/L ZnCP(6h) group. Cells were harvested, and the cell proteins were extracted to detect the changes in the expression of the apoptosis-related proteins caspase-3, Fas,, Bax, and Bcl-2 using Western Blot.Results:1.In human osteosarcoma MG-63 cells, clear bands of cleaved caspase-3 was observed;The Fas detection values in each group were,0.52±0.072?0.85±0.053? 1.58±0.071?1.72±0.207?1.02±0.033?1.34±0.182 respectively;and the Bax/Bcl-2 ratios in each group were,5.23±0.88?8.27±1.01?14.62±1.69?19.89±0.86?12.82±0.97? 7.08±1.38, respectively.2.In human osteosarcoma U-2 OS cells, clear bands of cleaved caspase-3 was observed;The Fas detection values in each group were,0.61±0.085? 0.98±0.53?1.58±0.236?1.97±0.177?1.60±0.401?1.38±0.236 respectively;and the Bax/Bcl-2 ratios in each group were,4.12±0.68?6.27±1.01?12.62±1.86?18.69±0.82? 12.41±0.95?6.01±0.88,respectively.3.There were statistically significant differences in cleaved caspase-3, Fas, and Bax/Bcl-2 ratio between the control group and groups 1,2, and 3 (P<0.05). There were statistically significant differences in cleaved caspase-3, Fas, Fas-L, and Bax/Bcl-2 ratio across groups 5,4 and 3 (P<0.05).Conclusion:ZnCP could induce apoptosis of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells, with significant time and concentration dependence. ZnCP might induce apoptosis of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells through the death receptor and mitochondrial pathways.?. Effects of a novel 3D ZnCP on the migration and invasion of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells and its mechanism of actionObjective:To explore the effects of a novel 3D ZnCP on the migration and invasion of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells and its potential molecular mechanism.Methods:1. Passage human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells were randomized into the blank control group, DMSO vehicle group, 10?mol/L ZnCP group,20?mol/L ZnCP group, and 40?mol/L ZnCP group. Wound-healing assay was used to detect the migration of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells.2. Passage human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells were randomized into the blank control group, DMSO vehicle group, 10?mol/L ZnCP group,20?mol/L ZnCP group, and 40?mol/L ZnCP group. Following incubation for 24h, Western Blot was used to detect the expression of the invasion-related proteins MMP-2 and MMP-9.Results:1.The migration rates of human osteosarcoma MG-63 cells were (70.3±6.1)%, (69.8±7.2)%, (55.2±4.9)%, (43.8±3.6)%, and (28.8±5.2)%, respectively;The migration rates of human osteosarcoma U-2 OS cells were (67.8±7.5)%, (66±5.2)%, (52.9±5.8)%, (41.2±5.6)%, and (25.8±5.3)%, respectively.2.The detection values of MMP-2 in human osteosarcoma MG-63 cells in each group were,0.62±0.051?0.57±0.049? 0.48±0.037.0.38±0.041?0.24±0.026,respectively;The detection values of MMP-9 in human osteosarcoma MG-63 cells in each group were,0.63±0.064?0.58±0.051? 0.47±0.35?0.37±0.46?0.21±0.019, respectively;The detection values of MMP-2 in human osteosarcoma U-2 OS cells in each group were,0.45±0.053?0.41±0.048? 0.31±0.27?0.19±0.022?0.08±0.0021 respectively;the detection values of MMP-9 in human osteosarcoma U-2 OS cells in each group were,0.42±0.038?0.39±0.043? 0.30±0.029?0.17±0.033?0.07±0.021, respectively; 3.In human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells, compared to the control group, there were no significant changes in cell migration rate, MMP-2 or MMP-2 expression in the DMSO vehicle group (P>0.05); compared to the vehicle group, there were statistically significant reductions in cell migration rate, MMP-2 and MMP-9 expression in 10?mol/L ZnCP group,20?mol/L ZnCP group, and 40?mol/L ZnCP group (P<0.05).Conclusion:ZnCP could reduce migration and invasion of human osteosarcoma MG-63 cells and human osteosarcoma U-2 OS cells, potentially by down-regulating the expression of the invasion-related proteins MMP-2 and MMP-9. |
PDF Full Text Request