Identification Of Gene Families Related To The Quality Formation Of Two Kinds Of Economic Crops In Anhui Province

Pear belongs to the genus Pomaeeae(Pyrus L.)of Rosaceae.The yield and cultivation area of Chinese pear is second only to citrus and apple,ranking the third white pear variety.Pyrus bretschneideri cv.Dangshan Su.,native to Dangshan County,Anhui Province,is the main export pear variety in China.Anhui Province is a major cotton producing province,and naturally colored cotton is deeply loved by the consumer due to its environmental protection.Color cotton can be divided into two categories:brown cotton and green cotton.It can synthesize and accumulate pigments during the development of cotton fiber and is the major source of naturally colored fibers.Brown cotton flavonoids are also closely related to resistance to pests and diseases.It can be seen that Dangshan Su pear and colored cotton are two characteristic economic crops in Anhui Province.Soluble solid content is an important indicator of pear fruit quality,and soluble sugar is an important component of soluble solids.Therefore,it is important to study the key genes regulating sugar metabolism in pear fruit to improve pear quality.Color diversity and stability are an important quality indicator for colored cotton fibers.We attempted to characterize the dynamic transport of flavonoids(proanthocyanidins and anthocyanins)and to study their relationship with different transcription factors.1)Sucrose synthase are encoded by a multiple small gene family,which have distinct and partially overlapping expression patterns.The identification of the genes encoding sucrose synthase is the first step towards understanding their physiological roles and involvement in different metabolic processes.In our study,we have identified sucrose synthase gene families and subsequently characterized in pear(Pyrus bretschneideri Rehd.)Additionally,we have verified the transcript data and shown that PbSS24 have a potential role in all the stages of pear fruit development,while PbSS15 and PbSS30 both are involved in pear senescence fruit development stage.2)Similarly,SBP-box genes encoded a family of plant-specific transcription factors(TFs),which are significant regulators of many biological processes,particularly transition phase,microsporogenesis,megasporogenesis,ripening of fruit,trichome development on sepals,stamen filament elongation and homeostasis.We report SBP-box genes evolutionary relationship and divergence expression,our results provided precise information of SBP-box genes that will be supportive for ongoing research on this important gene family in fruit plants,predominantly in pear.These outcomes are,however,noteworthy for further understanding of the molecular basis for Trans-acting factors relationship with proanthocyanidins and other flavonoids.3)Although the function of ZHD genes has exposed in Arabidopsis and some other model crops,their rule during fiber development and stress response in upland cotton remains largely unknown.G.hirsutum is one of the most valuable agricultural crop in the world and has been extensively studied at the developmental and physiological levels.Available complete Gossypium genomes make it possible to we examine genome-wide identification,transcriptomic difference,characterization/duplication and family size spanning a broad evolutionary scale in the Gossypium genomes.Here we reported the identification of ZHD genes using Gossypium genomes sequences,their characteristics,phylogenetic and syntenic analyses,gene duplicates,chromosomal location,and evolutionary mechanisms were subsequently analyzed.Through a combination of qRT-PCR and proanthocyanidins(PA)accumulation analyses in brown cotton fibers,we concluded that the candidate genes involved in early fiber development and fiber pigment synthesis include the following:GhZHD29,GhZHD35,GhZHD30,GhZHD31,GhZHD11,GhZHD27,GhZHD18,GhZHD15,GhZHD16,GhZHD22,GhZHD6,GhZHD33,GhZHD13,GhZHD5,and GhZHD23.This study delivers insights into the evolution of the GhZHD genes in brown cotton,serves as a valuable resource for further studies,and identifies the conditions necessary for improving the quality of brown cotton fiber.