Analysis Of MRNAs And LncRNAs In The Chicken Embryo Fibroblasts On NDV Infection

Newcastle disease virus?NDV?is an avian paramyxovirus that causes significant economic losses to the poultry industry worldwide,with variations in NDV pathogenicity due to differences in virulence between strains.In this study,transcriptional profiles were obtained from chick embryo fibroblasts?CEFs?infected with the highly virulent NDV Herts/33 strain or the nonvirulent LaSota strain using RNA-seq.This yielded 8,433 transcripts associated with NDV infection.This list of candidate genes was then further examined using GO and KEGG analyses.It showed a high enrichment in the areas of cellular components and metabolic processes,with the cellular components possibly being associated with NDV pathogenicity.Among these 8,433 transcripts,3,616 transcripts associated with interferon-stimulated genes?ISGs?were obtained;these transcripts are involved in metabolic processes,including protein phosphorylation and protein modification.These results provide further insight into the identification of genes involved in NDV infection.The global survey of changes in gene expression performed herein provides new insights into the complicated molecular mechanisms underlying virus and host interactions and will enable the use of new strategies to protect chickens against this virus.In addition,the lncRNA analysis of NDV infection has been performed in this study.The antisense lncRNA LNC₀00710,a key lncRNA molecule,was upregulated by NDV infection.LncRNA LNC₀00710 was with the tail of polyA and located in the nucleus.LncRNA LNC₀00710 was significantly up-regulated on NDV infection and its expression was increased with the increase of infection time.When expression of LNC₀00710 was knockdown using RNAi,the replication of NDV was increased.This indicated that lncRNA LNC₀00710 inhibited the replication of NDV.Therefore,it is speculated that LNC₀00710 plays an important role in the replication of NDV.However,the specific regulatory mechanism remains need to be further investigated.