The Study Of Mechanisms Of Cullin-1 Regulating Osteosarcoma Cell Proliferation And Metastasis

Background: There is no reliable marker available for early detection,diagnostic confirmation or disease prognosis of osteosarcoma.Cullin-1(CUL1)is a newly reported tumor-related gene,and we aimed to unravel its role in osteosarcoma.Methods: In vitro experiments were carried out to transfect osteosarcoma cells transfected with Cullin-1 expressing lentivirus and interference Cullin-1 SiRNA,and the proliferation rate of cells was detected by CCK8 method.And to detect the migration and invasion ability.In order to further understand the mechanism of Cullin-1 proliferation,migration and invasion in osteosarcoma.We measure the expression of P27 of osteosarcoma cell.We transfected the lentiviral osteosarcoma cells transfected with Cullin-1 expressing to interfere with the MMP-9 of SiRNA,and then tested the proliferation,migration and invasion ability.In vivo,the lentivirus transfected osteosarcoma cells transfected with Cullin-1 were injected into BCLB/C mice,and the volume and weight of the tumor were measured 6 weeks later.Results: In vivo and in vitro,Cullin-1 promotes the proliferation,invasion and metastasis of osteosarcoma cells.Cullin1 promotes the degradation of p27.High levels of CUL1 promote the progression of osteosarcoma by up regulation of MMP9 expressionConclusions: We find Cullin1 promotes proliferation of osteosarcoma cells by promoting p27 protein degradation.Our results also suggest that Cul1 regulates invasion and metastasis of osteosarcoma cells through the MMP-9 pathway.CUL1 might be an important marker and a therapeutic target for osteosarcoma.