| Objective To detect the expression of histone deacetylase 3(HDAC3)in cervical cancer tissue and investigate the inhibition of HDAC3 by Small Interference RNA(si RNA)on the effect of HDAC3 in Hela cell line of cervical cancer.Methods The expression of HDAC3 in 12 specimens of normal cervical tissues,21 specimens of moderate to severe cervical intraepithelial neoplasia and 64 specimens of cervical carcinoma were measured by reverse transcription-Polymerase Chain Reaction(RT-PCR)and immunohistochemistry(IHC).Using HDAC3 inhibitors Entinostat(MS-275),cervical cancer Hela cells,Siha cell as model,drug effects on cell viability,proliferation and apoptosis.RT-PCR and Western blotting were used to detect the expression of HDAC3 in Hela after transfected with si RNA and control RNA.Results The expression levels of HDAC3 m RNA in normal cerviacal epithelia,CINⅡ~Ⅲ and cervical cancer were(2.125±0.537)、(1.386±0.793)、(1.0±0.212)respectively.The rate of HDAC3 positive expression in normal cervical epithelia,CINⅡ~Ⅲ and cervical cancer were 8%(1/12),38%(8/21),94%(60/64)respectively.The results of IHC revealed that there were relationship between the expression of HDAC3 protein and the degree of tumor differntiation,myometrial invasion and lymph node metastasis.Significant down-regulation of the expression of m RNA and protein levels of HDAC3 was found following transfection of the HDAC3 si RNA.Ratios(0.923±0.028)vs(0.091±0.001)、(0.887±0.035)vs(0.093±0.005),the difference was statistically significant(P<0.05).Conclusions HDAC3 may play an important role in the course of cervical cancer.HDAC3 selective inhibitor MS-275 has inhibitory effect on Hela and Siha cells,this effect may be achieved by inhibiting the HDAC3.The in vitro transient transfection of synthesized HDAC3-si RAN on the Hela cell line can down-regulate the expression of m RNA and protein levels in the cells.si RNA is likely to be a new way to treating the cervical cancer. |
