Experimental Study On Herpes Simplex Virus Keratitis Recurrence Inhibited By Curcumin In Multiple Targets

Objective:to verify whether curcumin can inhibit the recurrence of herpes simplex keratitis,and to detect the express of COX-2,NF-?B and VEGF in this experiment.Methods:the primary HSK infection animal model was build.Six weeks later when the cornea returned transparent,HSK was induced by ultraviolet light B.1 days before radiation till the next 7 days,curcumin eye drops and acyclovir eye drops,were used with the treatment groups.The control groups also were established to remove other reasons affect the experimental results.The slit lamp was used to observe the cornea,and the cells were cultured with a corneal wipe to verify whether there was a virus replication.The mice were sacrificed in 4,7,10,14,21d after UV irradiation,respectively.The COX-2 and NF-kappa B and VEGF were detected in the cornea and trigeminal ganglion.Results:the recurrence rate of the groups treated with curcumin and acyclovir were decreased significantly compared with the blank control group,curcumin group recurrence between the two groups of corneal tissue rate than acyclovir group,but the difference was not statistically significant,and the recurrence of trigeminal ganglion in curcumin group rate than acyclovir group,difference there were significant differences.Conclusion:Curcumin and acyclovir can effectively inhibit the recurrence of herpes simplex keratitis in mice,and the effect is stronger than that of acyclovir in the trigeminal ganglion.At the same time,the three indexes of COX-2,NF-kappa B and VEGF were decreased in different degrees,which indicated that the inhibition of curcumin on these three targets played an important role in inhibiting the activation and replication of HSV-1.