| The occurrence and development of gastric cancer were regulated by many factors, with muitiple processes. In many diseases including cancers, miRNAs play an important role. miRNAs not only could act as cancer suppressor genes, but also oncogenes. Meanwhile, mutations and deficiency can occur in miRNAs, which will lead to the abnormal expression of related genes. miR-128b has been regonized as a cancer suppressor gene in ovarial cancer, breast cancer and lung cancer. However, the effect and prognostic value of miR-128b in gastric cancer was still poorly known. Therefore, to investigate the function of miR-128b and its targets in gastric cancer are very meaningful.Objective:The study was designed to investigate the expression of miR-128b and biological function in gastric cancer cells, and its expression correlated with the amount of TRIM29. At the same time, for further study gastric cancer and found that the mechanism of development of gastric cancer early diagnosis and late treatment-related molecular markers by exploring the expression level of miR-128b in gastric cancer tissue and its clinical significance in the treatment of gastric cancer.Method:We examined miR-128b and TRIM29 mRNA expression in three kind of gastric cells by RT-PCR analysis, respectively. Gastric cancer cell proliferation (BGC-823) was determined by MTT method after transfection of miR-128b mimics and TRIM29 siRNA. Cell cycle was detected by flow cytometry. To observe effect of miR-128b and TRIM29 on BGC-823 cell invasion and apoptosis by cell scratch and transwell assay. To valid the direct target function of miR-128b on TRIM29 by double fluorescence enzyme element assay. We analyzed the correlation of expression of miR-128b and clinical data.Results:The expression of miR-128b and TRIM29 was downregulated and upregulated strongly in gastric cancer, respectively. Low expression of miR-128b and high expression of TRIM29 both can inhibit gastric cancer cell proliferation, and promote apoptosis, weakened the migration and invasion ability as well(p<0.01). There was direct target negative relationship between miR-128b and TRIM29. In addition, the expression of miR-128b was downregulated with the increased gastric cancer clinical stage(p<0.01). MiR-128b expression in patients with lymph node metastasis was significantly lower than in patients with no lymph node metastasis (P<0.01). There was obvious relationship between the prognosis of gastric cancer patients and the expression of miR-128b in gastric cancer tissue:miR-128b expression is lower, the worse patients prognosis (P<0.01)Conclusion:miR-128b and TRIM29 expression was downregulated and upwnregulated in gastric cancer, respectively, and associated with gastric cancer clinical stage and lymph node metastasis. Overexpression of miR-128b can reduce TRIM29 expression, further inhibit gastric cancer cell grouth, invasion and induce the cells apoptosis. MiR-128-b expression in gastric cancer tissue is associated with the prognosis of patients. |
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