The Exosomes Secreted By Lung Cancer Cells In Radiation Therapy Promote Endothelial Cell Angiogenesis By Transferring MiR-23a

Objective: To investigate the effect of microRNA-23a transfered by exosome secreted from lung adenocarcinoma cells in radiation therapy on proliferation and migration of HUVEC and its mechanism.Methods:Set HEK293?lung adenocarcinoma A549 and H1299 cells as exosomes source, HUVEC as exosomes receptor cells, and the experimental groups were: HEK293 exosome group, adenocarcinoma cell exosome group, adenocarcinoma cell+ irradiation exosome group, knockdown miRNA-23a expression by microRNA inhibitor. The proliferation of HUVEC was detected by CCK8 and Ki67 staining, migration ability of HUVEC was compared by wound healing test and transwell test. The transcriptional level of microRNA-23a and PTEN were detected by real-time PCR, protein level of PTEN and phosphorylated AKT were detected by Western Blot to analysis the mechanism.Results:This study found that exosomes of lung adenocarcinoma cells can promote HUVEC proliferation and migration, after the lung adenocarcinoma cells were irradiated, this ability will be further enhanced. Real-time PCR showed that microRNA-23a expression in lung adenocarcinoma cells and its exosomes was up-regulated after irradiated. The upregulated microRNA-23a transferd by exosomes to HUVECs, then promote PTEN expression, which promote the phosphorylation of AKT and thus increase the ability of proliferation and migration of HUVEC.Conclusion:Radiation therapy promoted the expression of microRNA-23a in the exosomes of lung adenocarcinoma cells, and then inhibited PTEN/phosphorylated AKT pathway in HUVEC, thus promote angiogenesis.