| Objective:Dipsacus asperoides also known as Dipsacus, in this study, we will explore the role of Dipsacus in the spinal cord injury in rats and the mechanism.Methods:In the first part,54 adult SD rats were randomly divided into 3 groups:blank group, control group and experimental group, with 18 rats in each group. The experiment was carried out in three batches (n=6) according to the time points of 3d, 7d and 14d. The control group was treated with T9-10 laminectomy, the Allen method was used to treat the spinal cord, and the rats in the control group were given normal saline by intragastric administration every day. In the experimental group, T9-10 laminectomy was performed, and spinal cord attack was performed. Basso Beattie Bresnahan (BBB) motor function score was performed on the 3rd,7th and 14th day after operation. Rats were sacrificed and sacrificed. All the specimens were stained by Nissl's method. The morphology of the neurons was observed, and the percentage of stained area was analyzed and compared. Sections of the specimens were also immunostained for immunofluorescence and the expression levels of nerve growth factor BDNF and NT-3 were compared by measuring the fluorescence intensity. In the second part,20 SD rats were randomly divided into two groups,10 rats in each group, the control group and the experimental group respectively. The rats in the control group were treated with normal saline by intragastric administration, While the experimental group of rats after spinal cord attack, the daily use of Dipsacus extract gavage. All the rats were sacrificed at 1 week after operation. Myeloperoxidase (MPO) activity was detected in the spinal cord tissue and the area of syringomyelia was counted by HE staining. In the third part, we used the same experimental animal and grouping modeling method as the second part. RT-PCR and Weston-Blok were used to detect the expression of NF-?B p56 and IKK. All the experimental results were analyzed by SPSS 19.0 software. The data of the measurement data were expressed as mean ± standard deviation. One-way ANOVA was used to compare the two groups. The q test was used to compare the two groups. P<0.05 was regarded as the statistically significant standard.Results:In the first part, compared with the blank group, the BBB scores of the lower limbs of the rats in the control group and the experimental group were significantly decreased. There was no significant difference between the two groups (P> 0.05) (P <0.05). The score of the rats in the experimental group was higher than that in the control group at 7 days and 14 days. In the observation of Nissl body staining, we found that after the spinal cord strike, the experimental group and the control group of rat neurons is a gradual reduction of the process, after the percentage of staining area statistical comparison, in the postoperative 3d, the experimental group (P<0.05). There was no significant difference between the control group and the control group (P<0.05). The percentage of neuron stained area in the experimental group was significantly higher than that in the control group at the 7th and 14th day after operation). Fluorescence intensity in experimental group and control group was significantly higher than that in blank group (P<0.05). In the three time points, the fluorescence intensity of BDNF and NT-3 was significantly higher than that of control group The fluorescence intensity of the experimental group was higher than that of the control group, there was significant difference (P<0.05). In the second part, the level of MPO activity and the area of syringomyelia in the experimental group were significantly lower than those in the control group (P<0.05). In the third part, the RNA and protein levels of F-?B p65 and IKK in the experimental group were higher than those in the control group (P <0.05).Conclusion:Dipsacus extract can inhibit the activation of IKK/NF-?B p65 pathway, decrease the release of inflammatory factors and leukocyte infiltration, relieve the secondary spinal cord injury in rats after spinal cord injury, and up-regulate nerve growth The expression of BDNF, NT-3, promote neural repair and improve the motor function of rats. |
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