| The widespread fungus Aspergillus flavus is a common saprophyte and opportunistic pathogen that is capable of causing diseases in agriculture crops,producing toxic secondary metabolites,and bringing about health problem in animals and humans.Under favorite conditions,A.flavus produces aflatoxins,which are extremely hepatotixc,immunosuppressive,and antinutritional mycotoxins associated with both acute and chronic toxicity in human and animals,aflatoxin?AF?and many other secondary metabolites.A.flavus colonization in crops causes considerable economic losses because of reduced utilization and lower price of aflatoxin-contaminated grains.Quercetin?3,3',4',5,7-pentahydroxyflavone?could inhibit the development and AF production in A.flavus.However,the molecular mechanism of A.flavus in response to quercetin is not clear.In this study,an RNA-seq approach was applied to explore the mechanism of quercetin effect on A.flavus.The results are as follows:1.Quercetin affects the development and inhibits AF production in A.flavus.2.By RNA-seq analysis,11308 genes were found in the A.flavus and a total of 665 differentially expressed genes were identified?|log2 Fold change|>1 and Probability>0.8?.340 genes were up-regulated and 325 genes were down-regulated in the A.flavus under quercetin treatment.GO and pathways analysis for different expressed genes showed that differentially expressed genes were mainly involved in structural constituent of ribosome and ribosome and so on.3.Two differentially expressed genes related to the development and conidial formation of A.flavus were significantly down-regulated,including AFLA 083110 and AFLA044790.4.The expressed transcripts of most genes in the AF biosynthetic gene cluster in A.flavus showed no significant changes after treatment with quercetin and were expressed at low levels.The transcription regulator genes aflS in this cluster show significant difference expression relative to the sample without quercetin treatment. |
PDF Full Text Request