The Impact Of Electroacupuncture "Baihui" On Osteotylus Of Rat Tibial Fracture

BackgroundFracture is a common clinical disease. With domestic and foreign scholarin-depth understanding of fracture healing, we have begun explore ways topromote healing from all aspects. We have found the fact in the clinical workand literature, when limb fractures with brain damage, fracture healing isaccelerated and osteotylus gorwn excessivly.But the specific theoretical basisis still not clear. Electroacupuncture, as a traditional treatment of fractures,nonunion means, has got continuous improvement. With the integration ofvarious theoretical system of medicine and the progress of science andtechnology, the mechanism of action studies have aroused widespread concernand attention.PurposeEstablish SD rat tibial fracture models. Observe the change ofosteotylus through morphology, molecular biology, pathology, superfine pathology when using electroacupuncture on “baihui”, to understand thesustainability of the head electroacupuncture effects on fracture healing.Methods128healthy male SD rats were randomly divided into4groups--32normal groups (group A), the tibia fracture model group (group B),32electroacupuncture “baihui” and tibia fracture model group (group C),32moderate brain injury and tibia fracture model group (group D). Groups ofexperimental animals shall be put to death after the intervention, respectivelyin the7d,14d,21d and,28d. Observe the osteotylus change of shape, size,color etc. Under the X-ray film, calculate the osteotylus and statisticalcomparison by Perkins osteotylus volume formula. Slice partial osteotylusand observe pathological changes. Choose the soft tissue from tibia fractureanimal models, observe pathological changes under electron microscope.Using partial brain tissue specimens and osteotylus specimens, detect BMP-2、TGF-β1throught immunohistochemical method. Observe it undermicroscope (400times), calculate the integral optical density (IDO) of positiveexpression cells and detect the brain derived neurotrophic factor proteinthrough Western blotting, compare statistical differences.Results1Give corresponding intervention to tibial fracture models、electroacupuncture treatment models、 brain injury animal models. Theexperimental results show that group D’osteotylus is larger than group B andC(P <0.05).2Osteotylus tissue is observed under the microscope of400times: groupB shows the classical fracture healing; Group C’s osteotylus grows obviouslyfaster than group B; Group D’s osteotylus grows the fastest. 3Osteotylus tissue of the first week under the electron microscope: we cansee the fibroblasts, chondroblasts, mesenchymal cells, Osteogenesis cells andendothelial cells, etc. The fibroblasts and cmesenchymal cells of group D aremore active than other groups.4Specimens of osteotylus’s BMP-2immunohistochemical: the detectiveresults of group B in week1, week2, week3, week4are significantlydifferent from group A; The results of Group C in week1, week2, week3andweek4are significantly different from group A. The results of group c inweek1, week2are significantly different from group B; The results of GroupD in week1, week2, week3and week4are significantly different from groupA, and are obviously different from group B and group C in week1.5Specimens of osteotylus’s TGF-β1immunohistochemistry: The resultsof group B in week1, week2, week3, week4are significantly different fromgroup A; Group C in week1, week2, week3and week4are significantlydifferent from group A and are significantly different from group B in week1,week2; The results of Group D in week1, week2, week3and week4aresignificantly difference from group A, group B and group C.6Specimens of Brain tissue’s BMP-2immunohistochemistry: The resultsof group A and group B has no significant difference from group C; the resultsof Group D in week1, week2are significantly different from group A, groupB and group C.7Specimens of Brain tissue’s TGF-β1immunohistochemistry: Theresults of group A and group B has no significant difference; The results ofGroup C in week1, week2, week3and week4are significantly differentfrom group A; and are significantly different from group B in week2,3,4.The results of Group D have significant difference from group A, group B andgroup C in week1, week2, week3and week4.8Western blotting detection of Osteotylus of BDNF protein: The result of group C has significant difference from group B in week1, week2; the resultsof Group D have significant difference from group B in week1, week2.Conclusions:1Electroacupuncture “baihui” can promote healing of rats tibial fracture，not only on the volume of the osteotylus,but also on the time of recovering.2Electroacupuncture “baihui” can increase osteoclasts、osteogenesiscells、 fibroblasts and chondroblasts. This will promote the fracture healing.3Electroacupuncture “baihui” can promote all the stage of fracture healing.Form the result of the immunohistochemical, we can find the increasing ofOsteotylus of BMP-2in the early and TGF-β1in the late, which is maybeone of the reason of fracture healing acceleration.4Brain injury can cause the increasing of Osteotylus of BMP-2in theearly and TGF-β1in the late, which is similar to the electroacupuncture“baihui” of fracture healing acceleration.5Electroacupuncture “baihui” increases BDNF in Osteotylus in the early2weeks, which may be one of the reasons for fracture healing acceleration.