The Experimental Study On The Safety Of ShuangHuangLian Powder Injection Ultrasonic Atomization Inhaling

Objective:Through atomizing ShuangHuangLian powder injection solution to the Kunming mice, observe each group of mice lung biopsy, the body weight of mice, lung wet weight, the lung index as well as the lung tissue of mice CC16expression and serum HypIL-8content. Make a preliminary assessment of the security of ShuangHuangLian powder injection inhalation.Methods:Take healthy72, only two weeks kunming mice were randomly divided into blank group, the control group, dexamethasone group, ShuangHuangLian booster injection of low dose group (referred to as the low dose group, similarly hereinafter), middle dose group, high dose group, six groups. Blank group will not be any processing, the control group in the distilled water atomization, the rest of the group with different concentrations of ShuangHuangLian powder injection solution (low, medium and high dose group) aerosol inhalation. After30days according to weight the mice, put to death, according to take in mice lung wet weight, calculate each mouse lung index. Observe the lung tissue pathological airway structural changes. Take immunohistochemistry to detect the group the CC16content in the lung tissue of mice, take serum enzyme linked immunosorbent assay method (ELISA) for measuring content of HYP, IL-8.Results:Biopsy Tip:blank control group, dexamethasone group were a focal proliferation of fibrous tissue of mouse lung tissue with inflammatory cell infiltration; complete the rest of the bronchial wall, lumen rounded wall and smooth musclenormal thickness, the quality no inflammatory cell infiltration in the vessel lumen, alveolar space, lung. The Shuanghuanglian low-dose group:three mouse lung tissue focal fibrous tissue hyperplasia with inflammatory cell infiltration, and the rest no obvious abnormalities. Middle dose group:two mouse lung tissue focal fibrous tissue hyperplasia with inflammatory cell infiltration, and the rest showed no abnormalities. High dose group:two mouse lung tissue focal fibrous tissue hyperplasia with inflammatory cell infiltration, and the rest no obvious abnormalities. Mice in each group pathological classification, statistical analysis, the results were statistically significant (p<0.05).Experimental indicators, compared with the control group the dexamethasone group body weight of mice, lung wet weight was statistically significant (p<0.05); compared with the control group, each group of mice lung index was not statistically significant; blank group comparison, the high-dose group, CC16expression in the lung tissue of mice in the dexamethasone group was significantly higher statistically significant (p<0.05); middle dose group and low dose group, serum levels of dexamethasone Hyp content significantly higher, statistically significant (p<0.05); middle dose group and low dose group, the content of dexamethasone serum levels of IL-8was significantly higher (p<0.05) was statistically significant.Conclusion:In this experiment, ShuangHuangLian powder injection atomization inhalation, the lung tissue of mice have a certain damage. It is an alarming thing. The specific damage mechanism is worth further study. It is not come up with that ShuangHuangLian powder injection and dexamethasone, intravenous can be used for atomizing. The clinician must be in accordance with the drug instructions, and do not change the route of administration at random.