| Purpose：To analyze and excavate scutellaria potential functions which hasnot been specifically mentioned in ancient herbal but had a large number ofapplications in ancient prescriptions with database management system of “PujiFang”. While they were confirmed by experimental research, then we can clearand rich new features in traditional Chinese medicine. And demonstrated thescientificity and practicality of a new method to find new features oftraditional Chinese medicine.Material and method： Research includes literature research andexperimental research. In literature research ancient prescriptions and herbsliterature analysis method was used to investigate ancient herbal expositionsabout scutellariae function, and found that some features of scutellariaeancient herbal said didn’t match with medical standard of2010edition ChinesePharmacopoeia recording. In general, the real records of clinical applicationretained among successive prescriptions. Therefore, with database managementsystem of “Puji Fang”, using the ancient prescription database retrievaltechniques studied the application of scutellariae in ancient books.We confirmedscutellariae function. We introduced mathematical statistical analysis methodin order to confirm the potential function of scutellaria. In order to verifydiuresis and elimination of edema of scutellaria function which the ancientprescriptions and herbs book had discussed, we conducted experimental study.We established water load model in rats with the SD healthy male rats.Mice stomachwere perfused with2.5ml/kg saline, after the collection of urine within24h,the voided volume reached more than40%of the amount of gavage were selected intothe experiment.60qualified rats of pre-selection were randomly divided intomodel control group (referred to as the model group), scutellariae high dosegroup, scutellaria middle dose group, scutellariae low dose group, furosemidegroup, Wuling tablets group. After fasted for24hours but water, gastricperfusion of saline (5ml/100g) was given, mice lower abdomen were oppressed todrained enuresis, model group was given saline. The rats were placed inmetabolic cages (one rat per cage), recorded for the first time urination timesince the water load to rats as urination incubation period. Urine were collectedhourly, collected five hours at a stretch.Recorded per hour per only animal urineoutput and calculated total urinary amount, in order to preliminarily explorediuretic effect of scutellariae decoction, depth explord whether scutellariaedecoction have a therapeutic effect on nephritis. Experimental MsPGN model ofunilateral nephrectomized SD rats were induced with pre-immune and immunecombined method. In Modeling5th weekend using metabolic cages collected24hurine of rats, determined of24h urinary protein content of the rats in eachgroup with coomassie blue method.Excluding three urine protein negative ratsand24death rats in modeling process,the remaining61rats were randomly dividedinto model control group (referred to as the model group), scutellariae highdose group, scutellariae middle dose group, scutellariae low dose group,nephritis tablets group, prednisone tablets group, individually housed12ratswithout model for the control group (referred to as normal group). In Modeling6th weekend started to administrate while continued to carry out immunization.Model rats orally administrated once a day for6weeks.Collection of samplesfor analysis were conducted24hours after the last administration in mice ofeach group.Detected24-hour urinary protein and blood biochemical parameters,Observed the morphological changes of renal tissue in the light microscope,determinated serum levels of IL-1, TGF-β1and TNF-α with ELISA, DetectedMMP-9and NF-κB expression of renal tissue in rat by immunohistochemistry method,detected MMP-9mRNA and NF-ΚBp65mRNA expression of renal tissue by RT-PCR method.Results:1. We get scutellaria compound3737which were used to958kinds ofdiseases by retrieving. In ancient prescription books scutellaria was used totreat thoracic and abdominal pain, headache, body pain, and so on, we speculatedthat scutellaria may relieve pain. The scutellaria for panic epilepsy, epilepsywind, all epileptic seizures, epilepsy, hot epilepsy, as well as the treatmentof stroke, scutellaria may have function of calm the wind and stop the spasms.Scutellaria for the treatment of difficulty in urination and scutellaria foredema, all swollen, scutellaria may have the benefits of water swelling. Insummary we confirmed that ancient compound of containing scutellaria foredema,pain, neuropsychiatric disorders, stroke, consumption, all eye diseaseshad been discarded. Chi-square test statistics showed that ancient scutellariacompound treating edema,pain, neuropsychiatric disorders, stroke, consumption,all eye diseases had a very definite advantage, superior to scutellaria treatmentof fetal irritability which was recorded by medical standard of the2010editionChinese Pharmacopoeia.2. Improved the methods of arrange and analysis about ancient recipe.3.Scutellaria diuretic experimental results showed that urination incubationperiod of the drug treatment group had shortened to varying degrees, which wassignificantly different (p<0.05)comparing with the normal group, with astatistically significant. Comparing with scutellaria high dose group,urination incubation period of the scutellaria middle dose group and scutellarialow dose group were longer, significant differences (P<0.05), explained thatthe diuretic effect of scutellaria high dose group was superior to the othertwo groups. Comparing with the normal group, drug intervention groups5h urineoutput were significantly increased (P<0.05),5h urine output of scutellariahigh dose group were most, explained that diuretic effect of scutellaria highdose group was best.4. Experimental results of scutellaria treating MsPGN showed that24h urinary protein levels of the medication groups were significantly decreased(p<0.05)comparing with the model group. Among the three groups, the therapeuticeffect of scutellaria high dose group was best. After drug intervening, bloodfat, serum creatinine, blood urea nitrogen levels of all groups were reducedsignificantly, serum total protein was elevated, liver function did not changesignificantly. Kidney tissue pathological observation of model group showeddiffuse glomerular lesions and partial glomerular atrophy, an increasing thenumber of cells in the glomerular, the ECM severed gathered, widened mesangialarea, glomerular capillary cavity pressure change narrow, glomerular basementmembrane thickening, renal interstitial infiltration of inflammatory cellswhile all groups improved after drug treatment. Comparing with model group serumIL-1, TNF-α, TGF-β1expression of drug intervention groups decreasedsignificantly. NF-κB-p65expression of the model rats in the glomerular wassignificantly increased, and most of the brown particles had entered the nucleus,and had high expression in the nucleus. NF-κBp65of drug intervention groupspositively expressed mainly in the cytoplasm, although higher than the normalcontrol group, but significantly reduced (P<0.05)comparing with the model group.Comparing with the normal group the expression of MMP-9in the model group(p<0.05)significantly increased, and MMP-9expression of kidney tissue was notlimited to the tubular, expressed significantly in the glomerular also.Comparing with the model group, the drug intervention groups could decrease theexpression level of MMP-9, p<0.05, the expression level of MMP-9was the lowestin the scutellaria high dose group. Comparing with the model group, the drugintervention groups can significantly reduced the expression levels of NF-ΚBp65mRNA and MMP-9mRNA. Expression levels of NF-ΚBp65mRNA and MMP-9mRNA werelowest in scutellaria high dose group.Conclusion：1. Scutellaria has the potential function to treat edema, pain,neuropsychiatric disorders, stroke, consumption, all eye diseases. 2. The scutellaria decoction benefits of water swelling.3. The scutellaria decoction can reduce the urine protein content of MsPGN rats,protect the renal function, reduce glomerular injury, has a certain therapeuticeffect on MsPGN rats. The therapeutic mechanisms of scutellaria decoction forMsPGN rats may reduce the activation of NF-κBp65by inhibiting the expressionof inflammatory cytokines (IL-1, TNF-α, TGF-β1), thereby regulating theexpression of MMP-9.4. With database management system of “Puji Fang”, relying on ancientprescription database retrieval technology, combining with logical analysis andmodern statistical methods, then be confirmed by the experimental study, wefound that the method has scientific feasibility. |
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